{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:51:20Z","timestamp":1791165080328,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"abstract":"Abstract<\/jats:title>\n \n Background and Objectives<\/jats:title>\n To assess the effects of long\u2010term regular leukocyte\u2010reduced platelet (PLT) apheresis on lymphocyte subsets in volunteer donors.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n We enrolled 178 participants, including Amicus\u2010using donors (Group A,\n n<\/jats:italic>\n \u2009=\u200955), Trima\u2010using donors (Group T,\n n<\/jats:italic>\n \u2009=\u200964), and first\u2010time donor controls (\n n<\/jats:italic>\n \u2009=\u200959). Apheresis donors completed \u22658 PLT collections within 1\u2009year on one fixed separator. Lymphocyte\u2010subset measurements were performed on pre\u2010donation screening samples.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n After age\/sex adjustment, both apheresis groups exhibited significantly lower absolute counts and percentages of natural killer (NK)\u00a0cells versus controls (\n p<\/jats:italic>\n \u2009<\u20090.05), while CD3\n +<\/jats:sup>\n , CD4\n +<\/jats:sup>\n , CD8\n +<\/jats:sup>\n T cells, and B lymphocytes were similar among groups. Several T\u2010cell markers showed nominal downward trends with higher lifetime donations (raw\u2009\n p<\/jats:italic>\n \u2009<\u20090.05), yet no associations persisted after correcting for false discovery rate (FDR). Neither lifetime nor 1\u2010year donation counts correlated with lymphocyte or routine hematological parameters; overall lymphocyte\u2010subset profiles did not differ between Group A and Group T. Subgroup analysis revealed reduced NK cell counts only in the high\u2010donation stratum (\u2265100 donations) of Group\u2009A and the low\u2010donation stratum (<40 donations) of Group\u2009T.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Long\u2010term leukocyte\u2010reduced PLT apheresis is associated with decreased circulating NK cells. Multiple\u2010testing correction provided no evidence for progressive lymphocyte decline with cumulative donations. One\u2010year donation frequency had no impact on lymphocyte subsets. With plasma rinseback activated for all Trima procedures, lymphocyte profiles were comparable between devices. Routine hematological parameters remained stable in long\u2010term apheresis donors.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vox.70384","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:04:40Z","timestamp":1791162280000},"source":"Crossref","is-referenced-by-count":0,"title":["Monitoring of lymphocyte subsets in regular blood donors"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2448-6614","authenticated-orcid":false,"given":"Xiaoyan","family":"Yu","sequence":"first","affiliation":[{"name":"Beijing Red Cross Blood Center Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Red Cross Blood Center Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaru","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Red Cross Blood Center Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Beijing Red Cross Blood Center Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0889-8588(18)30110-2","article-title":"The use of leukocyte\u2010reduced blood components","volume":"9","author":"Miller JP","year":"1995","journal-title":"Hematol Oncol Clin North Am"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1002\/jca.2920010307","article-title":"Cellular depletion by apheresis","volume":"1","author":"Strauss RG","year":"1983","journal-title":"J Clin Apher"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1182\/blood-2018-09-873125","article-title":"Plateletpheresis\u2010associated lymphopenia in frequent platelet donors","volume":"133","author":"Gansner JM","year":"2019","journal-title":"Blood"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1111\/trf.16175","article-title":"Frequent platelet donation is associated with lymphopenia and risk of infections: a nationwide cohort study","volume":"61","author":"Zhao J","year":"2020","journal-title":"Transfusion"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1111\/trf.15441","article-title":"Severe CD4+ T cell lymphopenia is not observed in frequent plateletpheresis donors collected on the Fenwal Amicus","volume":"59","author":"Gansner JM","year":"2019","journal-title":"Transfusion"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1111\/trf.17567","article-title":"Risk factors for T\u2010cell lymphopenia in frequent platelet donors: the BEST collaborative study","volume":"63","author":"Kaufman RM","year":"2023","journal-title":"Transfusion"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1159\/000365425","article-title":"Blood donor management in China","volume":"41","author":"Shi L","year":"2014","journal-title":"Transfus Med Hemother"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1111\/trf.15192","article-title":"CD4+ T\u2010cell lymphopenia in frequent platelet donors who have ceased platelet donation for at least 1 year","volume":"59","author":"Rahmani M","year":"2019","journal-title":"Transfusion"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.xcrm.2022.100893","article-title":"The Fc\u2010effector function of COVID\u201019 convalescent plasma contributes to SARS\u2010CoV\u20102 treatment efficacy in mice","volume":"4","author":"Ullah I","year":"2023","journal-title":"Cell Rep Med"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1111\/trf.17037","article-title":"COVID\u201019 vaccine humoral response in frequent platelet donors with plateletpheresis\u2010associated lymphopenia","volume":"62","author":"Laumaea AE","year":"2022","journal-title":"Transfusion"},{"key":"e_1_2_9_12_1","first-page":"202","article-title":"Immunogenicity of quadrivalent meningococcal conjugate vaccine in frequent platelet donors","volume":"142","author":"Desjardins M","year":"2023","journal-title":"Blood"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1111\/tme.12208","article-title":"Improvement of plateletpheresis via technical modification on the MCS+","volume":"25","author":"Zhou Q","year":"2015","journal-title":"Transfus Med"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.transci.2020.102914","article-title":"The effects of autoflow management on flow\u2010rate alerts, collection efficiency, and collection rate during plateletpheresis","volume":"59","author":"Feng Q","year":"2020","journal-title":"Transfus Apher Sci"},{"key":"e_1_2_9_15_1","first-page":"114","article-title":"Leukocyte reduction filters are reliable and economic source for natural killer cell preparation","volume":"20","author":"Rezwani M","year":"2023","journal-title":"Iran J Immunol"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/978-1-60761-362-6_23","article-title":"Functional analysis of human NK cells by flow cytometry","volume":"612","author":"Bryceson YT","year":"2010","journal-title":"Methods Mol Biol"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"4912","DOI":"10.1182\/blood.V110.11.4912.4912","article-title":"Leukoreduction system chambers as a source of viable human peripheral blood mononuclear cells","volume":"110","author":"Kim S","year":"2007","journal-title":"Blood"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1699-0463.1992.tb00855.x","article-title":"Redistribution of lymphocytes after cortisol administration","volume":"100","author":"Toft P","year":"1992","journal-title":"APMIS"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1399-6576.1993.tb03708.x","article-title":"Redistribution of lymphocytes after major surgical stress","volume":"37","author":"Toft P","year":"1993","journal-title":"Acta Anaesthesiol Scand"}],"container-title":["Vox 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