{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:38:27Z","timestamp":1792546707764,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Maternal immunoglobulin G (IgG) anti\u2010A and anti\u2010B antibodies can cross the placenta and cause ABO haemolytic disease of the foetus and newborn (ABO\u2010HDFN). We aimed to evaluate whether maternal IgG anti\u2010A and anti\u2010B titres predict neonatal direct antiglobulin test (DAT) positivity in ABO\u2010incompatible pregnancies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>In this prospective observational cohort study, 889 consecutive ABO\u2010incompatible mother\u2013newborn pairs were enrolled between November 2025 and June 2026. Maternal IgG anti\u2010A and anti\u2010B titres were measured after 0.01\u2009M dithiothreitol treatment using the conventional tube antiglobulin titration method. Neonatal ABO grouping and DAT were performed by column agglutination technology. Receiver operating characteristic (ROC) curve analysis and multivariable logistic regression assessed the predictive performance of maternal antibody titres.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall, 112\/889 (12.6%) neonates were DAT positive. Maternal IgG anti\u2010A and anti\u2010B titres were significantly higher in mothers of DAT\u2010positive neonates (both\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Maternal IgG anti\u2010A (area under the curve [AUC] 0.778) and anti\u2010B (AUC 0.803) showed acceptable diagnostic performance. Higher thresholds (anti\u2010A \u226532; anti\u2010B \u226516) achieved 100% specificity, whereas lower thresholds (anti\u2010A \u22658; anti\u2010B \u22654) provided high sensitivity for excluding neonatal DAT positivity. Increasing maternal IgG anti\u2010A (adjusted odds ratio [aOR] 1.12, 95% confidence interval [CI]: 1.06\u20131.18) and anti\u2010B titres (aOR 1.18, 95% CI: 1.09\u20131.28) independently predicted DAT positivity (both\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Most DAT\u2010positive neonates required phototherapy (94.6%) but none required exchange transfusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Standardised maternal IgG anti\u2010A and anti\u2010B titres independently could predict neonatal DAT positivity. A dual\u2010threshold approach may complement existing antenatal assessment by identifying high\u2010risk pregnancies and guiding individualised postnatal surveillance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vox.70400","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:57:41Z","timestamp":1792544261000},"source":"Crossref","is-referenced-by-count":0,"title":["Maternal immunoglobulin G anti\u2010A and anti\u2010B antibody titres for predicting neonatal immune sensitisation in ABO\u2010incompatible pregnancies: The Maternal Anti\u2010A and Anti\u2010B Antibody Titres in ABO\u2010Incompatible Pregnancies study"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8643-2862","authenticated-orcid":false,"given":"Rajat","family":"Bansal","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, FMHS SGT University  Gurugram Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5999-7403","authenticated-orcid":false,"given":"Neerav","family":"Saini","sequence":"additional","affiliation":[{"name":"Department of Pathology, FMHS SGT University  Gurugram Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/vox.12265","article-title":"Haemolytic disease of the fetus and newborn","volume":"109","author":"Haas M","year":"2015","journal-title":"Vox Sang"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/s41390-020-01232-5","article-title":"Prediction of ABO hemolytic disease of the newborn using pre\u2010 and perinatal quantification of maternal anti\u2010A\/anti\u2010B IgG titer","volume":"90","author":"Krog GR","year":"2021","journal-title":"Pediatr 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hemolysis and hyperbilirubinemia by IgG subclass in blood group A or B infants born to group O mothers","volume":"123","author":"Kaplan M","year":"2009","journal-title":"Pediatrics"},{"key":"e_1_2_9_19_1","first-page":"69","article-title":"Immunological aspects of ABO haemolytic disease of the newborn","volume":"9","author":"Contreras M","year":"1980","journal-title":"Clin Haematol"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1097\/AOG.0b013e31817d453c","article-title":"Management of rhesus alloimmunization in pregnancy","volume":"112","author":"Moise KJ","year":"2008","journal-title":"Obstet Gynecol"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1054\/blre.1999.0123","article-title":"RhD haemolytic disease of the fetus and the newborn","volume":"14","author":"Urbaniak SJ","year":"2000","journal-title":"Blood Rev"}],"container-title":["Vox 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