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Current SLN mapping techniques can be costly and often require advanced imaging equipment. The objective of this prospective, method comparison study was to compare an SLN mapping protocol of lymphoscintigraphy to lymphography using water soluble iodinated contrast medium (WIC) and digital radiography for identification of an SLN. Lymphoscintigraphy and lymphography were performed on eight healthy purpose\u2010bred dogs using technetium\u201099m sulfur colloid and WIC injected into the subcutaneous tissues in a four\u2010quadrant technique around a predefined area of skin on the brachium. Images were obtained using a gamma camera and digital radiography at different time points post\u2010injection. Image sequences were evaluated by one of two American College of Veterinary Radiology board\u2010certified veterinary radiologists. Data obtained were compared between methods using descriptive statistics. An SLN was identified in all dogs with lymphoscintigraphy and seven of eight dogs with lymphography. Agreement between results of the lymphoscintigraphy and lymphography studies was a complete match in three dogs, a partial match in four dogs, and no match in one dog. The SLN detected differed based on the imaging modality used.<\/jats:p>","DOI":"10.1111\/vru.12908","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T14:30:38Z","timestamp":1601044238000},"page":"659-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sentinel lymph node detection differs when comparing lymphoscintigraphy to lymphography using water soluble iodinated contrast medium and digital radiography in dogs"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0234-5603","authenticated-orcid":false,"given":"Katelyn C.","family":"Hlusko","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Auburn University College of Veterinary Medicine  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Clinical 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