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In this descriptive and retrospective case series, ultrasonographic examination was performed on suspensory ligament branches with the limbs in weight bearing and non\u2010weight bearing positions. Suspensory ligament branch splits were defined as linear regions of decreased echogenicity when imaged with the limb in a weight bearing position that increased in size and became anechoic with the limb in a non\u2010weight bearing position. This appearance was considered an indication of pathologic change in the branch. A total of 62 suspensory ligament branches were included in the study from 37 horses, with 14 partial splits, 11 intrasubstance splits, and 14 complete splits of which two had extension of fetlock synovial fluid and synovial membrane through the split. Recheck ultrasonographic examinations performed in eight horses up to 14 months following the initial examination demonstrated persistence of the split in two horses, partial resolution in five horses, and complete resolution in one horse. Ultrasonographic examination using the non\u2010weight bearing approach proved valuable for increasing the lesion conspicuity as compared to the weight bearing images. Certain abnormalities, such as longitudinal fiber disruption (split) in suspensory ligament branches may only be evident when imaged non\u2010weight bearing. The use of this technique provides a more accurate representation of lesion severity and allows for monitoring over time. A longitudinal study is necessary to determine the clinical relevance of suspensory ligament branch splits.<\/jats:p>","DOI":"10.1111\/vru.12910","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T03:34:59Z","timestamp":1603337699000},"page":"84-97","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Non\u2010weight bearing ultrasonographic examination allows the diagnosis of longitudinal fiber disruption (split) in equine suspensory ligament branches not visible on weight bearing examination"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4230-7831","authenticated-orcid":false,"given":"Natasha","family":"Werpy","sequence":"first","affiliation":[{"name":"Equine Diagnostic Imaging Inc.  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