{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:32:18Z","timestamp":1770222738135,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T00:00:00Z","timestamp":1460678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the expression of biofilm\u2010associated genes in <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> on multiple clinically relevant surfaces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>In vitro experimental study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Sample Population<\/jats:title><jats:p>Two strains of methicillin\u2010resistant <jats:italic>S. pseudintermedius<\/jats:italic> isolated from clinical infections representing the most common international isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A quantitative polymerase chain reaction (qPCR) assay for expression of genes related to biofilm initial adhesion, formation\/maturation, antimicrobial resistance, and intracellular communication was developed and validated. <jats:italic>S. pseudintermedius<\/jats:italic> biofilms were grown on 8 clinically relevant surfaces (polymethylmethacrylate, stainless steel, titanium, latex, silicone, polydioxanone, polystyrene, and glass) and samples of logarithmic and stationary growth phases were collected. Gene expression in samples was measured by qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant differences in gene expression were identified between surfaces and between bacterial strains for most gene\/strain\/surface combinations studied. Expression of genes responsible for production of extracellular matrix were increased in biofilms. Expression of genes responsible for initial adhesion and intracellular communication was markedly variable. Antimicrobial resistance gene expression was increased on multiple surfaces, including stainless steel and titanium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A method for evaluation of expression of multiple biofilm\u2010associated genes in <jats:italic>S. pseudintermedius<\/jats:italic> was successfully developed and applied to the study of biofilms on multiple surfaces. Variations in expression of these genes have a bearing on understanding the development and treatment of implant\u2010associated biofilm infections and will inform future clinical research.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vsu.12471","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T10:48:37Z","timestamp":1460717317000},"page":"499-506","source":"Crossref","is-referenced-by-count":23,"title":["Biofilm\u2010Associated Gene Expression in <i>Staphylococcus pseudintermedius<\/i> on a Variety of Implant Materials"],"prefix":"10.1111","volume":"45","author":[{"given":"Evan C.","family":"Crawford","sequence":"first","affiliation":[{"name":"Department of Clinical Studies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameet","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas W.G.","family":"Gibson","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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