{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T15:35:57Z","timestamp":1784302557527,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T00:00:00Z","timestamp":1512691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the efficacy of repeated decontamination and sterilization of a disposable port intended for 1\u2010time use during single\u2010incision laparoscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>Experimental; prospective, controlled design.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six single\u2010access ports used 4 times and 6 single\u2010access ports used 8 times to perform various clean, minimally invasive surgeries were evaluated. Ports were decontaminated in an enzymatic cleaner (dilution, 3:100) and cleaned with a scrub brush for 5 minutes. The ports were then sterilized with hydrogen peroxide vapor for 50 minutes using a standard protocol at a concentration of 6 mg\/L, followed by a vapor diffusion phase. Samples taken from the foam, insufflating tubing, and rigid cannula portion of each port were collected with aseptic technique for aerobic\u2013anaerobic cultures. Port material samples were set up on a tryptic soy agar plate with 5% sheep blood, a MacConkey agar plate, and a Columbia agar plate with 5% sheep blood (CAP). Anaerobic isolate cultures were set up on Centers for Disease Control and Prevention (CDC) blood agar and CAP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the ports used 4 times had positive bacteriologic culture. Two of the ports used 8 times had a light growth of bacteria. The first positive sample cultured <jats:italic>Staphylococcus<\/jats:italic> spp. and <jats:italic>Micrococcus<\/jats:italic> spp. The second positive sample cultured <jats:italic>Staphylococcus epidermidis<\/jats:italic>. The positive cultures were obtained from the foam component in an enriched broth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Single\u2010incision ports could be used safely 4 times and pose a low risk of infection to the patient under conditions of this study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vsu.12761","type":"journal-article","created":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T10:46:39Z","timestamp":1512729999000},"source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of efficacy of repeated decontamination and sterilization of single\u2010incision laparoscopic surgery ports intended for 1\u2010time use"],"prefix":"10.1111","volume":"47","author":[{"given":"Brian","family":"Petrovsky","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine Colorado State University Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Monnet","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine Colorado State University Fort Collins Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004640000118"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/109264202760268023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-011-1884-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-001-9190-7"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.74.6.934"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-11692011000200020"},{"key":"e_1_2_6_8_1","volume-title":"Making Health Care Safer II: An Updated Critical Analysis of the Evidence for Patient Safety Practices","author":"Noble M.","year":"2013"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30496"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-11692010000100013"},{"key":"e_1_2_6_11_1","first-page":"823","article-title":"Safety evaluation of single\u2010use medical devices after submission to simulated reutilization cycles","volume":"88","author":"da Silva MV","year":"2005","journal-title":"J AOAC Int."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.100060"},{"key":"e_1_2_6_13_1","unstructured":"Covidien. 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ISO\/TS 15883\u20105:2005(en). Washer\u2010disinfectors\u2014Part 5: Test soils and methods for demonstrating cleaning efficacy.https:\/\/www.iso.org\/obp\/ui\/#iso:std:iso:ts:15883:\u20105:ed\u20101:v1:en. Accessed November 16 2017."},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2008.03.002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00464-004-8112-x"},{"key":"e_1_2_6_31_1","unstructured":"United States Government Accountability Office. Reprocessed single\u2010use medical devices: FDA oversight has increased and available information does not indicate that use presents an elevated health risk. Report to the Committee on Oversight and Government Reform House of Representatives [GAO\u201008\u2010147]. Washington DC: United States Government Accountability Office; Jan 2008.http:\/\/www.gao.gov\/new.items\/d08147.pdf. Accessed November 16 2017."},{"key":"e_1_2_6_32_1","first-page":"81","article-title":"To reuse or not to reuse? 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