{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T11:18:34Z","timestamp":1784546314077,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T00:00:00Z","timestamp":1573603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the effect of storage temperature on cadaveric small intestinal leakage pressures after enterotomy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>Experimental ex vivo study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Grossly normal jejunal segments from four canine cadavers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010six jejunal segments (n = 12 segments\/group) were harvested immediately after euthanasia and assigned to a fresh group (tested within 4\u2009hours), chilled group (stored for 24\u2009hours at 4\u00b0C before testing), or freeze\u2013thaw group (frozen at \u221220\u00b0C for 7\u2009days and thawed at 21\u00b0C for 6\u2009hours before testing). A 2\u2010cm antimesenteric enterotomy was performed and repaired with 4\u20100 monofilament suture in a simple\u2010continuous pattern. Initial leakage pressure (ILP), maximal intraluminal pressure (MIP), and leakage location were recorded, with testing performed at room temperature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean\u2009\u00b1\u2009SD ILP for fresh, chilled, and frozen\u2013thawed specimens was 52.9\u2009\u00b1 8.4, 51.8\u2009\u00b1\u200911.9 and 29.8\u2009\u00b1\u20094.4\u2009mm Hg, respectively. There was a difference in ILP among groups (<jats:italic>P\u2009&lt;<\/jats:italic> .003), with freeze\u2013thaw samples demonstrating lower ILP compared with other groups. There was no difference in MIP between groups (<jats:italic>P<\/jats:italic> = .186) There was a difference in leakage location among groups (<jats:italic>P\u2009=<\/jats:italic> .004), with the majority of chilled and freeze\u2013thaw samples leaking at the suture holes compared with the incisional line in fresh samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Freezing and subsequent thawing prior to specimen testing reduced ILP compared with use of fresh and chilled specimens but did not affect MIP among experimental groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical significance<\/jats:title><jats:p>Cadaveric canine intestinal specimens tested immediately after collection or after chilling for 24\u2009hours should be recommended for ex vivo burst pressure assessment in dogs. Additional studies to evaluate loss in testing viability of chilled intestinal specimens are warranted to help govern experimental methodologies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vsu.13339","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T12:19:48Z","timestamp":1573647588000},"page":"496-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Ex vivo comparison of the effect of storage temperature on canine intestinal leakage pressures"],"prefix":"10.1111","volume":"49","author":[{"given":"Daniel J.","family":"Duffy","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Jen","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie A.","family":"Balko","sequence":"additional","affiliation":[{"name":"Department of Molecular and Biomedical Sciences, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George E.","family":"Moore","sequence":"additional","affiliation":[{"name":"Veterinary Administration, College of Veterinary Medicine Purdue University  West Lafayette Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,13]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1513","volume-title":"Veterinary Surgery: Small Animal","author":"Brown 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