{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:05:58Z","timestamp":1791950758745,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2022\u201067015\u201036351"],"award-info":[{"award-number":["2022\u201067015\u201036351"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to study the antimicrobial and pro\u2010healing potential of equine mesenchymal stromal cell secreted products (i.e. secretome), collected as conditioned media (mesenchymal stromal cell\u2010conditioned media, MSC CM), in a novel in vivo model of methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA)\u2010inoculated equine thorax wounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>Prospective in vivo study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Animals<\/jats:title><jats:p>Two Thoroughbred geldings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six full\u2010thickness cutaneous wounds were created bilaterally on the dorsal thorax of two horses (<jats:italic>n<\/jats:italic>\u2009=\u200912 wounds\/horse). Wounds on the left thoraces were inoculated with MRSA on day 0. All wounds were then treated with either mupirocin ointment, MSC CM, or vehicle control (<jats:italic>n<\/jats:italic>\u2009=\u20094 wounds per group) once daily for 3\u2009days. Photographs were taken to quantify wound scores and sizes, as well as samples to determine bacterial colony forming units (CFUs), at days 0, 1, 2, 3, 7, 14, 21, and 28. The wound edge was biopsied on days 0, 7, and 28, and scored histologically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Inoculated wounds had more bacterial CFUs at day 1 (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.0001) and were larger in size at day 28 (<jats:italic>p<\/jats:italic>\u2009=\u2009.0009) than noninoculated wounds. Mupirocin\u2010treated wounds were smaller than MSC CM and vehicle control\u2010treated wounds at day 28 (<jats:italic>p<\/jats:italic>\u2009=\u2009.003). Mesenchymal stromal cell\u2010conditioned media did not affect CFU numbers in inoculated and noninoculated wounds. Moreover, MSC CM did not affect the parameters of wound size or gross or microscopic wound scores over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mesenchymal stromal cell\u2010conditioned media did not exhibit antimicrobial or pro\u2010healing properties in the current study; however, the in vivo model of inoculated equine thorax wounds requires further optimization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical significance<\/jats:title><jats:p>This pilot study contributes to a growing understanding of the equine MSC secretome as an antimicrobial and pro\u2010healing therapeutic for equine wounds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vsu.14170","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T07:13:35Z","timestamp":1728112415000},"page":"1377-1389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preliminary in vivo investigation of the mesenchymal stromal cell secretome as a novel treatment for methicillin\u2010resistant <i>Staphylococcus aureus<\/i> in equine skin wounds"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9317-9235","authenticated-orcid":false,"given":"Megan J.","family":"Fahey","sequence":"first","affiliation":[{"name":"Baker Institute for Animal Health, Department of Microbiology and Immunology Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8715-2562","authenticated-orcid":false,"given":"Rebecca M.","family":"Harman","sequence":"additional","affiliation":[{"name":"Baker Institute for Animal Health, Department of Microbiology and Immunology Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9039-5689","authenticated-orcid":false,"given":"Matthew A.","family":"Thomas","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Department of Clinical Sciences Cornell University  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1636-2439","authenticated-orcid":false,"given":"Brenna R.","family":"Pugliese","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Department of Clinical Sciences Cornell University  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9610-9040","authenticated-orcid":false,"given":"Jeanine","family":"Peters\u2010Kennedy","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Department of Population Medicine and Diagnostic Sciences Cornell University  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0973-2075","authenticated-orcid":false,"given":"Michelle L.","family":"Delco","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Department of Clinical Sciences Cornell University  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2064-8029","authenticated-orcid":false,"given":"Gerlinde R.","family":"Van de Walle","sequence":"additional","affiliation":[{"name":"Baker Institute for Animal Health, Department of Microbiology and Immunology Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/VR.105880"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/VMS3.1073"},{"key":"e_1_2_9_4_1","unstructured":"LehenbauerTW SmithDR FajtVR et al.AVMA\/Committee on Antimicrobials: Antimicrobial Resistant Pathogens Affecting Animal Health in the United States.2020. 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