{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:38:26Z","timestamp":1790145506176,"version":"4.0.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T00:00:00Z","timestamp":1364256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Abstract<\/jats:title>Venous ulcers are related to dysfunctions in extracellular matrix. Both matrix metalloproteinases (MMP<\/jats:styled-content>) and neutrophil gelatinase\u2010associated lipocalin (NGAL<\/jats:styled-content>) could play a role in the healing process in patients with chronic venous ulcers. We evaluated the role of MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> in the healing process in venous ulceration. We performed an open\u2010label, parallel groups, single clinical center study. Patients with chronic venous leg ulcers represented the test group (Group I<\/jats:styled-content>), whereas patients without chronic ulcers represented the control group (Group II<\/jats:styled-content>). In Group I<\/jats:styled-content> plasma and wound fluid samples were collected at the time of admission, at the time of the surgery, and at the follow\u2010up, while ulcer tissues were taken at the time of the surgery. In Group II<\/jats:styled-content>, plasma and wound fluid were collected at admission and at the time of the surgery, whereas skin tissues were collected at the time of the surgery. Enzyme\u2010linked immunosorbent assay test was used to evaluate the levels of MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> in plasma and wound fluid, whereas Western blot analysis was performed to estimate the expression of MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> in tissues. Enzyme\u2010linked immunosorbent assay tests revealed significantly higher levels of MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> in both plasma and wound fluid of patients with ulcers compared to patients without ulcers (p<\/jats:italic>\u2009<\u20090.01). Moreover, Western blot analysis documented an increased expression of MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> in biopsy tissue of patients with ulcers compared to patients without ulcers (p<\/jats:italic>\u2009<\u20090.01). In conclusion MMP<\/jats:styled-content>\u20109 and NGAL<\/jats:styled-content> may correlate with the clinical course of venous ulcers.<\/jats:p>","DOI":"10.1111\/wrr.12035","type":"journal-article","created":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T16:10:49Z","timestamp":1364314249000},"page":"395-401","source":"Crossref","is-referenced-by-count":109,"title":["Chronic venous leg ulcers are associated with high levels of metalloproteinases\u20109 and neutrophil gelatinase\u2010associated lipocalin"],"prefix":"10.1111","volume":"21","author":[{"given":"Raffaele","family":"Serra","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"Buffone","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Falcone","sequence":"additional","affiliation":[{"name":"Department of Health Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Molinari","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Scaramuzzino","sequence":"additional","affiliation":[{"name":"Department of Health Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Gallelli","sequence":"additional","affiliation":[{"name":"Department of Health Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"de Franciscis","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Science School of Medicine University Magna Gracia of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.avsg.2011.04.008"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sder.2005.10.002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.avsg.2011.11.036"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.avsg.2012.03.016"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30218-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2010.05.003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2010.09.001"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2011.05.012"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08462.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2318-10-S1-A64"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2004.09.027"},{"key":"e_1_2_6_13_1","first-page":"213","article-title":"Effects of simvastatin and rosuvastatin on RAS, matrix metalloproteinases and NF\u2010kB in lung cancer and in normal pulmonary tissues","volume":"48","author":"Falcone D","year":"2012","journal-title":"Cell Prolif"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200204"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20884"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2008.00538.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463201002300209"},{"key":"e_1_2_6_18_1","first-page":"25","article-title":"Effects of statins and farnesyl transferase inhibitors on ERK phosphorylation, apoptosis and cell viability in non small lung cancer cells","volume":"11","author":"Pelaia G","year":"2012","journal-title":"Cell Prolif"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.20.6832"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20608"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20124"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH06-11-0638"}],"container-title":["Wound Repair and Regeneration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fwrr.12035","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.12035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T17:50:17Z","timestamp":1698515417000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/wrr.12035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,26]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1111\/wrr.12035"],"URL":"https:\/\/doi.org\/10.1111\/wrr.12035","archive":["Portico"],"relation":{},"ISSN":["1067-1927","1524-475X"],"issn-type":[{"value":"1067-1927","type":"print"},{"value":"1524-475X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,26]]}}}