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For venous leg ulcers and diabetic foot ulcers, a bilayered living cellular construct (<jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content>), containing both fibroblasts and keratinocytes that participate in cross talk, is a safe and effective product in healing chronic wounds. To show the importance of both cell types in <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content>, constructs were generated containing only fibroblasts or only keratinocytes and compared directly to <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content> via histology, mechanical testing, gene\/protein analysis, and angiogenesis assays. <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content> contained a fully differentiated epithelium and showed greater tensile strength compared with one\u2010cell\u2010type constructs, most likely due to formation of intact basement membrane and well\u2010established stratum corneum in <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content>. Furthermore, expression of important wound healing genes, cytokines, and growth factors was modulated by the cells in <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content> compared with constructs containing only one cell type. Finally, conditioned medium from <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content> promoted greater endothelial network formation compared with media from one\u2010cell\u2010type constructs. Overall, this study characterized a commercially available wound healing product and showed that the presence of both fibroblasts and keratinocytes in <jats:styled-content style=\"fixed-case\">BLCC<\/jats:styled-content> contributed to epithelial stratification, greater tensile strength, modulation of cytokine and growth factor expression, and increased angiogenic properties compared with constructs containing fibroblasts or keratinocytes alone.<\/jats:p>","DOI":"10.1111\/wrr.12154","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T16:09:03Z","timestamp":1395072543000},"page":"246-255","source":"Crossref","is-referenced-by-count":212,"title":["The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing"],"prefix":"10.1111","volume":"22","author":[{"given":"Abigail M.","family":"Wojtowicz","sequence":"first","affiliation":[{"name":"Research and Development Department Organogenesis, Inc.  Canton Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Research and Development Department Organogenesis, Inc.  Canton Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Carlson","sequence":"additional","affiliation":[{"name":"Research and Development Department Organogenesis, Inc.  Canton Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agatha","family":"Zawadzka","sequence":"additional","affiliation":[{"name":"Preclinical Department Organogenesis, Inc.  Canton Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecile F.","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Preclinical Department Organogenesis, Inc.  Canton Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dolores","family":"Baksh","sequence":"additional","affiliation":[{"name":"Research and Development Department Organogenesis, Inc.  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