{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T01:17:56Z","timestamp":1784510276669,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,7,18]],"date-time":"2014-07-18T00:00:00Z","timestamp":1405641600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1DP2 DK083085-01"],"award-info":[{"award-number":["1DP2 DK083085-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recurrent injury has been implicated in the development of chronic diabetic wounds. We have developed a chronic diabetic wound model based upon recurrent injury in diabetic mice. We hypothesized that dysregulation of collagen production at both the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">microRNA<\/jats:styled-content> levels contributes to the development of chronic diabetic wounds. To test this, both diabetic and nondiabetic mice were made to undergo recurrent injury. Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\"><jats:italic>TGF<\/jats:italic><\/jats:styled-content><jats:italic>\u2010\u03b21<\/jats:italic>, <jats:styled-content style=\"fixed-case\"><jats:italic>SMAD<\/jats:italic><\/jats:styled-content><jats:italic>\u20103<\/jats:italic>, <jats:italic>Col1\u03b11<\/jats:italic>, <jats:italic>Col3\u03b11<\/jats:italic>, <jats:styled-content style=\"fixed-case\">microRNA<\/jats:styled-content>\u201025, and <jats:styled-content style=\"fixed-case\">microRNA<\/jats:styled-content>\u201029a and Western blot for collagen <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> were performed 7 days following each injury. Diabetic wounds displayed decreased collagen at all time points. This was associated with dysregulated collagen production at both the gene and <jats:styled-content style=\"fixed-case\">microRNA<\/jats:styled-content> levels at all time points. Following the final injury, however, diabetic collagen production significantly improved. This appeared to be due to a substantial decrease in both <jats:styled-content style=\"fixed-case\">microRNAs<\/jats:styled-content> as well as an increase in the expression of collagen pathway genes. That dysregulated collagen production progressed throughout the course of wounding suggests that this is one factor contributing to the development of chronic diabetic wounds. Future studies using this model will allow for the determination of other factors that may also contribute to the development and\/or persistence of chronic diabetic wounds.<\/jats:p>","DOI":"10.1111\/wrr.12199","type":"journal-article","created":{"date-parts":[[2014,6,5]],"date-time":"2014-06-05T08:18:49Z","timestamp":1401956329000},"page":"515-520","source":"Crossref","is-referenced-by-count":31,"title":["Dysregulation of collagen production in diabetes following recurrent skin injury: Contribution to the development of a chronic wound"],"prefix":"10.1111","volume":"22","author":[{"given":"Robert C.","family":"Caskey","sequence":"first","affiliation":[{"name":"Department of General Surgery University of Pennsylvania School of Medicine  Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Zgheib","sequence":"additional","affiliation":[{"name":"Department of Surgery Nemours Children's Hospital  Orlando 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