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In this project, the stromal cell\u2010derived factor 1 (SDF<\/jats:styled-content>\u20101)\/C\u2010X\u2010C chemokine receptor (CXCR<\/jats:styled-content>) 4<\/jats:styled-content> and SDF<\/jats:styled-content>\u20101\/CXCR7<\/jats:styled-content> pathway were focused in human adipose\u2010derived stem cells (ASC<\/jats:styled-content>s). ASC<\/jats:styled-content>s were incubated with acute (AWF<\/jats:styled-content>) or chronic wound fluid (CWF<\/jats:styled-content>) to analyze their effects by quantitative real\u2010time polymerase chain reaction (SDF<\/jats:styled-content>\u20101, CXCR4<\/jats:styled-content>, CXCR7<\/jats:styled-content>, TIMP3<\/jats:styled-content>), enzyme\u2010linked immunosorbent assay (SDF<\/jats:styled-content>\u20101 in WF<\/jats:styled-content>s and supernatant), and transwell migration assay with\/without antagonization. Whereas SDF<\/jats:styled-content>\u20101 amounted 73.5\u2009pg\/mL in AWF<\/jats:styled-content>, it could not be detected in CWF<\/jats:styled-content>. Incubation with AWF<\/jats:styled-content> led to a significant enhancement (129.7\u2009pg\/mL vs. 95.5\u2009pg\/mL), whereas CWF<\/jats:styled-content> resulted in a significant reduction (30\u2009pg\/mL vs. 95.5\u2009pg\/mL) of SDF<\/jats:styled-content>\u20101 in ASC<\/jats:styled-content> supernatant. The SDF<\/jats:styled-content>\u20101 receptor CXCR7<\/jats:styled-content> was detected on ASC<\/jats:styled-content>s. AWF<\/jats:styled-content> but not CWF<\/jats:styled-content> significantly induced ASC<\/jats:styled-content> migration, which was inhibited by CXCR4<\/jats:styled-content> and CXCR7<\/jats:styled-content> antagonists. Expressions of SDF<\/jats:styled-content>\u20101, CXCR4<\/jats:styled-content>, and CXCR7<\/jats:styled-content> were significantly stimulated by AWF<\/jats:styled-content> while TIMP3<\/jats:styled-content> expression was reduced. In conclusion, an uncontrolled inflammation in the chronic wound environment, indicated by a reduced SDF<\/jats:styled-content>\u20101 expression, resulted in a decreased ASC<\/jats:styled-content> migration. A disturbed SDF<\/jats:styled-content>\u20101\/CXCR4<\/jats:styled-content> as well as SDF<\/jats:styled-content>\u20101\/CXCR7<\/jats:styled-content> pathway seems to play an important role in the impaired healing of chronic wounds.<\/jats:p>","DOI":"10.1111\/wrr.12248","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T06:51:38Z","timestamp":1421045498000},"page":"82-89","source":"Crossref","is-referenced-by-count":39,"title":["The role of SDF<\/scp>\u20101 in homing of human adipose\u2010derived stem cells"],"prefix":"10.1111","volume":"23","author":[{"given":"Ewa K.","family":"Stuermer","sequence":"first","affiliation":[{"name":"Institute for Research in Operative Medicine (IFOM) University of Witten\/Herdecke Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Lipenksy","sequence":"additional","affiliation":[{"name":"Clinic for Plastic and Reconstructive Surgery, Handsurgery Burn Care Center 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