{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T09:32:41Z","timestamp":1771925561516,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Programs Foundation of Education Department of Liaoning Province","award":["L2015506"],"award-info":[{"award-number":["L2015506"]}]},{"name":"National High Technology Research and Development Program of China","award":["2012AA020905"],"award-info":[{"award-number":["2012AA020905"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral nerve injury is an ongoing challenge in reconstructive surgery. Adipose\u2010derived stem cell (ADSC) application is reported to improve nerve regeneration. In the present study, we evaluated the potential benefit of 34a\u2010ADSCs for never regeneration. Lentiviral vectors containing miRNA\u201034a were constructed and ADSCs were transduced. The obtained 34a\u2010ADSCs were used to regenerate the sciatic nerve in surgically induced sciatic nerve injury rat model. Sprague\u2013Dawley (SD) rats were randomly divided into two groups, a 34a\u2010ADSC group and an Lv\u2010ADSC group. Functional nerve recovery was assessed by walking track analysis at 12 weeks after surgery. In addition, histology, including light microscopy and transmission electron microscopy, and immunohistochemistry, was utilized to investigate the nerve repair effects of 34a\u2010ADSC. Results showed that reconstruction of the injured sciatic nerve had been significantly enhanced by restoration of nerve continuity and functional recovery in the 34a\u2010ADSC group compared with the Lv\u2010ADSC group. Furthermore, sciatic nerve conduction velocity and compound nerve action potential in the 34a\u2010ADSC group was much higher than that in the Lv\u2010ADSC group (30.72\u2009\u00b1\u20092.95 m\/s vs. 22.73\u2009\u00b1\u20091.91 m\/s, <jats:italic>p<\/jats:italic>&lt; 0.0001; 11.93\u2009\u00b1\u20090.76 mV vs. 9.52\u2009\u00b1\u20090.53 mV, <jats:italic>p<\/jats:italic>\u2009=\u20090.0418). This study raises the possibility of using miRNA\u201034a overexpressed ADSCs as a promising alternative for nerve regeneration.<\/jats:p>","DOI":"10.1111\/wrr.12427","type":"journal-article","created":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T09:02:47Z","timestamp":1456131767000},"page":"542-550","source":"Crossref","is-referenced-by-count":11,"title":["Transplantation of miRNA\u201034a overexpressing adipose\u2010derived stem cell enhances rat nerve regeneration"],"prefix":"10.1111","volume":"24","author":[{"given":"Xingliang","family":"He","sequence":"first","affiliation":[{"name":"Key Lab of School of Kinesiology Shenyang Sport University and"},{"name":"Department of Tissue Engeering China Medical University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Ao","sequence":"additional","affiliation":[{"name":"Department of Tissue Engeering China Medical University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujun","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Tissue Engeering China Medical University Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinrui","family":"Song","sequence":"additional","affiliation":[{"name":"Key Lab of School of Kinesiology Shenyang Sport University 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