{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:18:06Z","timestamp":1790680686228,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,8]],"date-time":"2016-06-08T00:00:00Z","timestamp":1465344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["#DK098055\u201006A1"],"award-info":[{"award-number":["#DK098055\u201006A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Current chronic wound treatments often fail to promote healing of diabetic foot ulcers (DFU), leading to amputation and increased patient morbidity. A critical mediator of proper wound healing is the production, assembly, and remodeling of the extracellular matrix (ECM) by fibroblasts. However, little is known about how these processes are altered in fibroblasts within the DFU microenvironment. Thus, we investigated the capacity of multiple, primary DFU\u2010derived fibroblast strains to express, produce, and assemble ECM proteins compared to diabetic patient\u2010derived fibroblasts and healthy donor\u2010derived fibroblasts. Gene expression microarray analysis showed differential expression of ECM and ECM\u2010regulatory genes by DFU\u2010derived fibroblasts which translated to functional differences in a 3D in vitro ECM tissue model. DFU\u2010derived fibroblasts produced thin, fibronectin\u2010rich matrices, and responded abnormally when challenged with transforming growth factor\u2010beta, a key regulator of matrix production during healing. These results provide novel evidence that DFU\u2010derived fibroblasts contribute to the defective matrices of DFUs and chronic wound pathogenesis.<\/jats:p>","DOI":"10.1111\/wrr.12437","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T21:57:43Z","timestamp":1461362263000},"page":"630-643","source":"Crossref","is-referenced-by-count":121,"title":["Altered ECM deposition by diabetic foot ulcer\u2010derived fibroblasts implicates fibronectin in chronic wound repair"],"prefix":"10.1111","volume":"24","author":[{"given":"Anna G.","family":"Maione","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avi","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Kashpur","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Yanez","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elana","family":"Knight","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Mooney","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering Harvard University Boston Massachusetts"},{"name":"School of Engineering and Applied Sciences Harvard University Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aristidis","family":"Veves","sequence":"additional","affiliation":[{"name":"Microcirculation Laboratory and Joslin\u2010Beth Israel Deaconess Foot Center Beth Israel Deaconess Medical Center, Harvard Medical School Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjana","family":"Tomic\u2010Canic","sequence":"additional","affiliation":[{"name":"Department of Dermatology &amp; Cutaneous Surgery, Wound Healing and Regenerative Medicine Research Program University of Miami Miller Medical School Miami Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Garlick","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Oral Medicine and Craniofacial Pain Tufts University School of Dental Medicine Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(91)70242-T"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12472373"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12499839"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.15"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00381.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1350-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1995.tb01109.x"},{"key":"e_1_2_7_9_1","first-page":"1085","article-title":"Sequential changes in histologic pattern and extracellular matrix deposition during the healing of chronic venous ulcers","volume":"141","author":"Herrick SE","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016068917266"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07039"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62953-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.1992.tb03514.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.12.2407"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2011.01.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12329920"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00549.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5309.75"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2006.06.034"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000065640.77499.D7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2014.0414"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-011-9478-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","unstructured":"GK Smyth 2005 397 420","DOI":"10.1515\/9783110928822.bm"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-5-282"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-48"},{"key":"e_1_2_7_26_1","first-page":"103","article-title":"Collagen gel contraction assay","volume":"341","author":"Ngo P","year":"2006","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4161\/15592294.2014.967584"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00547.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(97)00622-1"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060161"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002614"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750060601"}],"container-title":["Wound Repair and Regeneration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fwrr.12437","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.12437","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T04:21:20Z","timestamp":1695010880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/wrr.12437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,8]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/wrr.12437"],"URL":"https:\/\/doi.org\/10.1111\/wrr.12437","archive":["Portico"],"relation":{},"ISSN":["1067-1927","1524-475X"],"issn-type":[{"value":"1067-1927","type":"print"},{"value":"1524-475X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,8]]}}}