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In our work, we propose to investigate the effects of calcitriol as well as of a vitamin D analog (paricalcitol) and a calcimimetic (cinacalcet), in fin regeneration and de novo mineralization in a zebrafish model of diabetes. Following exposure of diabetic transgenic Tg(<jats:italic>ins:nfsb<\/jats:italic>\u2010mCherry) zebrafish to calcitriol, paricalcitol and cinacalcet, caudal fins were amputated to assess their effects on tissue regeneration. Caudal fin mineralized and regenerated areas were quantified by in vivo alizarin red staining. Quantitative real\u2010time PCR was performed using RNA from the vertebral column. Diabetic fish treated with cinacalcet and paricalcitol presented increased regenerated and mineralized areas when compared with non\u2010treated diabetic group, while no significant increase was observed in non\u2010diabetic fish treated with both drugs. Gene expression analysis showed an up\u2010regulation for runt\u2010related transcription factor 2b <jats:italic>(runx2b)<\/jats:italic>, bone gamma\u2010carboxyglutamic acid\u2010containing protein <jats:italic>(bglap)<\/jats:italic>, insulin a <jats:italic>(insa)<\/jats:italic> and insulin b <jats:italic>(insb)<\/jats:italic> and a trend of increase for sp7 transcription factor <jats:italic>(sp7)<\/jats:italic> in diabetic groups treated with cinacalcet and paricalcitol. Expression of <jats:italic>insra<\/jats:italic> and <jats:italic>vdra<\/jats:italic> was up\u2010regulated in <jats:italic>both<\/jats:italic> diabetic and nondiabetic fish treated with cinacalcet. In nondiabetic fish treated with paricalcitol and cinacalcet a similar increase in gene expression could be observed but not so pronounced. The increased mineralization and regeneration in diabetic zebrafish treated with cinacalcet and paricalcitol can be explained by increased osteoblastic differentiation and increased insulin expression indicating pro\u2010osteogenic potential of both drugs.<\/jats:p>","DOI":"10.1111\/wrr.12536","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T23:54:21Z","timestamp":1491436461000},"page":"432-442","source":"Crossref","is-referenced-by-count":15,"title":["Improved regeneration and de novo bone formation in a diabetic zebrafish model treated with paricalcitol and cinacalcet"],"prefix":"10.1111","volume":"25","author":[{"given":"Filipe R.","family":"Carvalho","sequence":"first","affiliation":[{"name":"Center of Marine Sciences (CCMAR) Faro Portugal"},{"name":"PhD Program in Biomedical Sciences University of Algarve Faro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Medicine University of Algarve Faro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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