{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:09:56Z","timestamp":1760346596372,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:00:00Z","timestamp":1494892800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR066755"],"award-info":[{"award-number":["R01AR066755"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Cultured epidermal autografts have been used worldwide since 1981 for patients with extensive third\u2010degree burn wounds and limited skin donor sites. Despite significant progress in techniques toward improving clinical outcome of skin grafts, the long in vitro preparation time of cultured autografts has remained a major factor limiting its widespread use. Here, we show that pharmacological inhibition of TGF\u2010\u03b2 signaling promotes the expansion of human epidermal keratinocytes (HEKs) with high proliferative potential in co\u2010cultures with both murine 3T3\u2010J2 cells and human feeder cells, including dermal fibroblasts and preadipocytes. In contrast, TGF\u2010\u03b2 signaling inhibition does not enhance the growth of HEKs in a serum\u2010 and feeder\u2010free condition, an alternative approach to propagate HEKs for subsequent autograft production. Our results have important implications for the use of TGF\u2010\u03b2 signaling inhibition as a viable therapeutic strategy for improving Green's methodology and for more efficient production of customized skin autografts with human feeder cells.<\/jats:p>","DOI":"10.1111\/wrr.12541","type":"journal-article","created":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T23:52:45Z","timestamp":1493077965000},"page":"526-531","source":"Crossref","is-referenced-by-count":7,"title":["Inhibition of TGF\u2010\u03b2 signaling promotes expansion of human epidermal keratinocytes in feeder cell co\u2010culture"],"prefix":"10.1111","volume":"25","author":[{"given":"Daisuke","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology Boston University Henry M. Goldman School of Dental Medicine Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipa","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Boston University Henry M. Goldman School of Dental Medicine Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0155-0683","authenticated-orcid":false,"given":"Makoto","family":"Senoo","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology Boston University Henry M. 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