{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:22:05Z","timestamp":1768519325601,"version":"3.49.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T00:00:00Z","timestamp":1507680000000},"content-version":"vor","delay-in-days":40,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31670996"],"award-info":[{"award-number":["31670996"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Shaanxi Province Natural Science Foundation","doi-asserted-by":"publisher","award":["2013JQ4022"],"award-info":[{"award-number":["2013JQ4022"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glycans are known to play important roles in molecular recognition, cell\u2013cell adhesion, molecular trafficking, receptor activation, and signal transduction during development and regeneration. Despite numerous investigations of regenerating salamander limbs, global analysis of the precise variation of glycans during the limb regeneration process has received little attention. Here, we have used lectin microarrays and lectin histochemistry to analyze the alterations and distribution of glycans during the early stages leading to blastema formation during <jats:italic>Cynops orientalis<\/jats:italic> limb regeneration in response to limb amputation. Compared with the control group, analysis at several time points (3, 7, and 14 days postamputation) using microarrays containing 37 lectins showed that limb tissues expressed significantly different complements of glycans recognized by 9 different lectins. Postamputation limb tissues showed higher expression of two glycan structures recognized by the lectins STL and LTL and lower expression of seven glycan structures recognized by PHA\u2010E, MAL\u2010I, SNA, UEA\u2010I, PHA\u2010E\u2009+\u2009L, VVA, and GNA. We also observed significant changes in glycans specifically at 7 days postamputation, including higher binding capacity by WFA, GSL\u2010I, and NPA and lower binding capacity by PNA, HHL, ConA, LCA, GSL\u2010II, and PWM. Next, we validated our lectin microarray data using lectin histochemistry in limb tissues. Glycans recognized by STL and GNA showed similar changes in signal intensity to those found in the lectin microarrays, with STL staining in the cytoplasm and GNA in the cytoplasm and nucleus. Our findings are the first report of significant postamputation changes in glycans in limb tissues and suggest that those glycans perform potentially important functions during the early stages of <jats:italic>C. orientalis<\/jats:italic> limb regeneration.<\/jats:p>","DOI":"10.1111\/wrr.12580","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T11:43:59Z","timestamp":1504179839000},"page":"836-845","source":"Crossref","is-referenced-by-count":7,"title":["Profiling of glycan alterations in regrowing limb tissues of <i>Cynops orientalis<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"Jihong","family":"Cui","sequence":"first","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"},{"name":"Provincial Key Laboratory of Biotechnology of Shaanxi  Xi'an 710069 People's Republic of China"},{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanxue","family":"Zheng","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyuan","family":"Jia","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yalong","family":"Feng","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmin","family":"Li","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"},{"name":"Provincial Key Laboratory of Biotechnology of Shaanxi  Xi'an 710069 People's Republic of China"},{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fulin","family":"Chen","sequence":"additional","affiliation":[{"name":"Lab of Tissue Engineering, College of Life Sciences Northwest University  Xi'an 710069 People's Republic of China"},{"name":"Provincial Key Laboratory of Biotechnology of Shaanxi  Xi'an 710069 People's Republic of China"},{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education  Xi'an People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9667"},{"key":"e_1_2_1_3_1","volume-title":"Limb regeneration","author":"Tsonis PA.","year":"1996"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.73"},{"key":"e_1_2_1_5_1","first-page":"887","article-title":"Vertebrate limb regeneration and the origin of limb stem cells","volume":"46","author":"Bryant SV","year":"2002","journal-title":"Int J Dev 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