{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T08:02:18Z","timestamp":1770451338663,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T00:00:00Z","timestamp":1518739200000},"content-version":"vor","delay-in-days":107,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004368","name":"Colgate-Palmolive Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004368","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We explored use of the suction\u2010blister wound model in the assessment of not only epidermal regeneration but also pain, the microvascular response and bacteriology. The effects of topical zinc sulfate were studied to articulate the methodologies in this double\u2010blind trial. One epidermal suction blister (10 mm) was induced on each buttock in 30 healthy volunteers (15 females:15 males) and deroofed on day 0. The wounds were randomized to daily treatment with 1.4% zinc sulfate shower gel (<jats:italic>n<\/jats:italic>\u2009=\u200920), placebo (<jats:italic>n<\/jats:italic>\u2009=\u200920) or control (<jats:italic>n<\/jats:italic>\u2009=\u200920). Digital photography coupled with planimetry, transepidermal water loss (TEWL) measurement and optical coherence tomography (OCT) was benchmarked to the gold standard of histology of 60 full\u2010thickness wound biopsies on day 4. Pain increased after application of the shower gels. Microvessel density, determined from OCT images, increased from day 0 to day 2 in the three groups but increased more with the placebo than with the zinc shower gel (<jats:italic>p<\/jats:italic>\u2009=\u20090.003) or the control treatment (<jats:italic>p<\/jats:italic>\u2009=\u20090.002) and correlated (<jats:italic>r<\/jats:italic><jats:sub>S<\/jats:sub>\u2009=\u20090.313, <jats:italic>p<\/jats:italic>\u2009=\u20090.015) with the inflammatory response on day 4, as determined by histology. Coagulase\u2010negative staphylococci were more common in wounds compared with skin (<jats:italic>p<\/jats:italic>\u2009=\u20090.002) and was reduced (<jats:italic>p<\/jats:italic>\u2009=\u20090.030) with zinc sulfate treatment. Planimetric analysis of digital wound images was not biased (<jats:italic>p<\/jats:italic>\u2009=\u20090.234) compared with histology, and TEWL measurements showed no correlation (<jats:italic>r<\/jats:italic><jats:sub>S<\/jats:sub>\u2009=\u20090.052, <jats:italic>p<\/jats:italic>\u2009=\u20090.691) with epithelialization. Neoepidermal formation, determined by histology, did not differ (<jats:italic>p<\/jats:italic>\u2009=\u20090.290) among the groups. Zinc sulfate reduced (<jats:italic>p<\/jats:italic>\u2009=\u20090.031) the release of lactate dehydrogenase from cultured gel\u2010treated keratinocytes isolated from the blister roofs. Therefore, combination of the standardized suction\u2010blister wound model with noninvasive planimetry and OCT is a useful tool for assessing wound therapies. Zinc sulfate transiently dampened inflammation and reduced bacterial growth.<\/jats:p>","DOI":"10.1111\/wrr.12605","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T05:51:33Z","timestamp":1515563493000},"page":"984-993","source":"Crossref","is-referenced-by-count":20,"title":["Noninvasive measurement of reepithelialization and microvascularity of suction\u2010blister wounds with benchmarking to histology"],"prefix":"10.1111","volume":"25","author":[{"given":"Heidi F.","family":"Larsen","sequence":"first","affiliation":[{"name":"Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malin G.","family":"Ahlstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lise M. R.","family":"Gjerdrum","sequence":"additional","affiliation":[{"name":"Department of Pathology Zealand University Hospital Roskilde Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette","family":"Mogensen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khaled","family":"Ghathian","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hvidovre Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Calum","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hvidovre Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne L.","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Section of Biostatistics, Department of Public Health University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie L.","family":"Forman","sequence":"additional","affiliation":[{"name":"Section of Biostatistics, Department of Public Health University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Vandeven","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Piscataway New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian N.","family":"Holerca","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Piscataway New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Du\u2010Thumm","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Piscataway New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars N.","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"Digestive Disease Center, Bispebjerg Hospital University of Copenhagen Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2263-0826","authenticated-orcid":false,"given":"Magnus S.","family":"\u00c5gren","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Copenhagen Wound Healing Center, Bispebjerg Hospital University of Copenhagen Copenhagen Denmark"},{"name":"Digestive Disease Center, Bispebjerg Hospital University of Copenhagen Copenhagen 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