{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:33:07Z","timestamp":1791426787130,"version":"4.3.3"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,5,19]],"date-time":"2018-05-19T00:00:00Z","timestamp":1526688000000},"content-version":"vor","delay-in-days":79,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005627","name":"Universit\u00e9 de Li\u00e8ge","doi-asserted-by":"publisher","award":["Fonds Sp\u00e9ciaux \u2010 Cr\u00e9dit classique 0000005312"],"award-info":[{"award-number":["Fonds Sp\u00e9ciaux \u2010 Cr\u00e9dit classique 0000005312"]}],"id":[{"id":"10.13039\/501100005627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003134","name":"Fonds pour la Formation \u00e0 la Recherche dans l\u2019Industrie et dans l\u2019Agriculture","doi-asserted-by":"publisher","award":["0000009232"],"award-info":[{"award-number":["0000009232"]}],"id":[{"id":"10.13039\/501100003134","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["Cr\u00e9dit de recherche 0000006539"],"award-info":[{"award-number":["Cr\u00e9dit de recherche 0000006539"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["Mandat chercheur qualifi\u00e9 0000009017"],"award-info":[{"award-number":["Mandat chercheur qualifi\u00e9 0000009017"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The zebrafish is a popular animal model with well\u2010known regenerative capabilities. To study regeneration in this fish, the nitroreductase\/metronidazole\u2010mediated system is widely used for targeted ablation of various cell types. Nevertheless, we highlight here some variability in ablation efficiencies with the metronidazole prodrug that led us to search for a more efficient and reliable compound. Herein, we present nifurpirinol, another nitroaromatic antibiotic, as a more potent prodrug compared to metronidazole to trigger cell\u2010ablation in nitroreductase expressing transgenic models. We show that nifurpirinol induces robust and reliable ablations at concentrations 2,000 fold lower than metronidazole and three times below its own toxic concentration. We confirmed the efficiency of nifurpirinol in triggering massive ablation of three different cell types: the pancreatic beta cells, osteoblasts, and dopaminergic neurons. Our results identify nifurpirinol as a very potent prodrug for the nitroreductase\u2010mediated ablation system and suggest that its use could be extended to many other cell types, especially if difficult to ablate, or when combined pharmacological treatments are desired.<\/jats:p>","DOI":"10.1111\/wrr.12633","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T04:48:31Z","timestamp":1523940511000},"page":"238-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Nifurpirinol: A more potent and reliable substrate compared to metronidazole for nitroreductase\u2010mediated cell ablations"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4175-8558","authenticated-orcid":false,"given":"David","family":"Bergemann","sequence":"first","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Massoz","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordane","family":"Bourdouxhe","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio A.","family":"Carril Pardo","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne L.","family":"Voz","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Peers","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Manfroid","sequence":"additional","affiliation":[{"name":"Zebrafish Development and Disease Models Laboratory\/GIGA University of Li\u00e8ge, Liege, 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