{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:31:56Z","timestamp":1778283116594,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003662","name":"Korea Evaluation Institute of Industrial Technology","doi-asserted-by":"publisher","award":["#10047890"],"award-info":[{"award-number":["#10047890"]}],"id":[{"id":"10.13039\/501100003662","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003624","name":"Ministry of Agriculture, Food and Rural Affairs","doi-asserted-by":"publisher","award":["#71500307"],"award-info":[{"award-number":["#71500307"]}],"id":[{"id":"10.13039\/501100003624","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002642","name":"Korea University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002642","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Proliferative and migratory abilities of fibroblasts are essential for wound healing at the skin surface. Cytoplasmic linker\u2010associated protein\u20102 (CLASP2) was originally found to interact with cytoplasmic linker protein (CLIP)\u2010170. CLASP2 plays an important role in microtubule stabilization and the microtubule\u2010stabilizing activity of CLASP2 depends on its interactions with end binding (EB)\u20101 and CLIP\u2010170. Although the microtubule\u2010stabilizing role of CLASP2 is well established, the effects of CLASP2 on the migration and proliferation of fibroblasts remain unclear in the context of wound healing. Therefore, we tested the utilization of CLASP2 as a directly applied protein drug to improve wound healing by promoting the migration of effector cells, including skin fibroblasts, to the site of repair or injury using an <jats:italic>in vivo<\/jats:italic> excisional wound mouse model and <jats:italic>in vitro<\/jats:italic> Hs27 skin fibroblast model. Epidermal growth factor, which is a recognized contributor to cell proliferation and migration, was used as positive control. <jats:italic>In vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, CLASP2 treatment significantly enhanced cell migration and accelerated wound closure. Furthermore, <jats:italic>in vivo<\/jats:italic>, the CLASP2\u2010treated animal group displayed enhanced epidermal repair and collagen deposition. Next, we studied the mechanism of CLASP2 for wound healing. Increasing the abundance of intracellular free CLASP2 in skin fibroblasts by supplying exogenous CLASP2 seemed to stabilize microtubules through an interaction between CLASP2 and CLIP\u2010170, as well as EB1. Exogenous CLASP2 also showed direct binding with IQGAP1, increasing both cyclic adenosine monophosphate activity and phosphorylation of glycogen synthase kinase 3\u03b2, which in turn reinstated the binding between free CLASP2 and IQGAP1. In summary, exogenous CLASP2 increased Hs27 skin fibroblast migration by interacting with IQGAP1 and other cytoskeletal linker proteins, such as CLIP\u2010170 and EB1. Our results strongly suggest that CLASP2 can be developed in wound healing drugs for skin repair and\/or regenerating cosmetic products.<\/jats:p>","DOI":"10.1111\/wrr.12713","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T04:10:21Z","timestamp":1551845421000},"page":"345-359","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Exogenous CLASP2 protein treatment enhances wound healing <i>in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"MiJung","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Life Sciences College of Life Sciences and Biotechnology, Korea University  145 Anam\u2010dong, Sungbuk\u2010ku, Seoul 136701 South Korea"},{"name":"Department of Biotechnology College of Life Sciences and Biotechnology, Korea University  145 Anam\u2010dong, Sungbuk\u2010ku, Seoul 136701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You\u2010Sun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Life Sciences and Biotechnology, Korea University  145 Anam\u2010dong, Sungbuk\u2010ku, Seoul 136701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Mi","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Life Sciences and Biotechnology, Korea University  145 Anam\u2010dong, Sungbuk\u2010ku, Seoul 136701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong\u2010Jin","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology School of Medicine, Konkuk University  120 Neungdong\u2010ro, Gwangjin\u2010gu, Seoul 143\u2010701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha\u2010Na","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology School of Medicine, Konkuk University  120 Neungdong\u2010ro, Gwangjin\u2010gu, Seoul 143\u2010701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changhee","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology School of Medicine, Konkuk University  120 Neungdong\u2010ro, Gwangjin\u2010gu, Seoul 143\u2010701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Min","family":"Yu","sequence":"additional","affiliation":[{"name":"R&amp;D Division CHA Biotech Co. 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