{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:36:11Z","timestamp":1783017371789,"version":"3.54.6"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T00:00:00Z","timestamp":1664496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ATP is a crucial molecule for every energy\u2010dependent process in cells. In ischemic tissues, ATP production declines, and it finally results in cell death. One of the most common strategies in burn wound management is saving the zone of ischemia. In the current study, Mg\u2010ATP\u2010containing nanoliposomes were formulated and studied in vitro and in vivo. The particle size of the vesicles was between 50 and 100\u2009nm and the mean zeta potential was \u22124.05\u2009\u00b1\u20090.52\u2009mV as evaluated by dynamic light scattering and Zeta sizer instrument, respectively. The encapsulation efficiency of ATP in the nanoliposomes was found to be 9.3%. The morphology and size of nanoliposomes were further studied by transmission electron microscopy. The standard MTT assay revealed no cytotoxicity of the nanoliposomes when tested on the rat fibroblast cells. Forty rats were randomly divided into four groups (<jats:italic>N<\/jats:italic>\u00a0=\u200910 each). Burn wounds were created by burn comb model on the back of the rats and the zone of stasis in each group was treated every 12\u2009h for 3\u2009days by injecting them with the Mg\u2010ATP\u2010nanoliposomes. Control samples included empty nanoliposomes, unencapsulated Mg\u2010ATP and the Krebs\u2013Henseleit buffer. Laser Doppler flowmetry results revealed that blood perfusion in the zone of ischemia in rats treated with Mg\u2010ATP\u2010nanoliposomes was more than in the other groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05). Histopathology revealed saving zone of stasis by Mg\u2010ATP\u2010nanoliposomes. Findings obtained in this study demonstrated that the formulated Mg\u2010ATP\u2010nanoliposome has the potential to save the stasis zone in burn wounds.<\/jats:p>","DOI":"10.1111\/wrr.13048","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T06:04:00Z","timestamp":1663221840000},"page":"28-39","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Saving zone of stasis in burn wounds by nanoliposomal <scp>Mg\u2010ATP<\/scp>"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-9748","authenticated-orcid":false,"given":"Farzad","family":"Hayati","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Faculty of Veterinary Medicine Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mehdi","family":"Ghamsari","sequence":"additional","affiliation":[{"name":"Department of 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