{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:18:25Z","timestamp":1770891505041,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypertrophic scars (HTS) develop from an excessive synthesis of structural proteins like collagen and a decreased expression of proteoglycans such as decorin. Previous research has demonstrated that decorin expression is significantly down\u2010regulated in HTS, deep dermal tissue, and thermally injured tissue, reducing its ability to regulate pro\u2010fibrotic transforming growth factor\u2010beta 1 (TGF\u2010\u03b21) and normal fibrillogenesis. However, treatment of HTS fibroblasts with interferon\u2010alpha 2b (IFN\u2010\u03b12b) has been shown to reduce excessive collagen synthesis and improve HTS by reducing serum TGF\u2010\u03b21 levels. The expression of decorin isoforms in HTS is currently unknown and the effects of TGF\u2010\u03b21 and IFN\u2010\u03b12b on decorin, decorin isoform expression and type 1 collagen are of great interest to our group. Dermal fibroblasts were treated with TGF\u2010\u03b21 and\/or IFN\u2010\u03b12b, for 48\u2009h. The expression and secretion of decorin, decorin isoforms and type 1 collagen were quantified with reverse transcription\u2010quantitative polymerase chain reaction, immunofluorescence staining and enzyme\u2010linked immunosorbent assays. The mRNA expression of decorin and each isoform was significantly reduced in HTS fibroblasts relative to normal skin. TGF\u2010\u03b21 decreased the mRNA expression of decorin and decorin isoforms, whereas IFN\u2010\u03b12b showed the opposite effect. IFN\u2010\u03b12b significantly inhibited TGF\u2010\u03b21's effect on the mRNA expression of type I collagen alpha 1 in papillary dermal fibroblasts and overall showed relative effects of inhibiting TGF\u2010\u03b21. These data support that a further investigation into the structural and functional roles of decorin isoforms in HTS pathogenesis is warranted and that IFN\u2010\u03b12b is an important agent in reducing fibrotic outcomes.<\/jats:p>","DOI":"10.1111\/wrr.13155","type":"journal-article","created":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T06:49:55Z","timestamp":1705733395000},"page":"135-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The effects of <scp>TGF\u2010\u03b21<\/scp> and <scp>IFN\u2010\u03b12b<\/scp> on decorin, decorin isoforms and type I collagen in hypertrophic scar dermal fibroblasts"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8527-3694","authenticated-orcid":false,"given":"Elizabeth E.","family":"Eremenko","sequence":"first","affiliation":[{"name":"Department of Surgery Faculty of Medicine &amp; Dentistry, University of Alberta  Edmonton Alberta 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