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Significant damage results in structure destruction and vision impairment. While extensive research has aimed at the restoring the corneal endothelial layer, including endothelial proliferation for functional monolayers remains challenging. Our previous studies confirmed the proliferative activity of stem cells from apical papilla\u2010conditioned medium (SCAP\u2010CM) on the retinal pigmented epithelium as a single cell layer. This study investigates how SCAP\u2010CM influences the proliferation and migration of CECs. Our results introduced Matrigel, as a new matrix component for in vitro culture of CECs. Moreover, 60% of SCAP\u2010CM was able to stimulate CEC proliferation as well as migrate to repair wound healing during 24\u2009h. Confluent CECs also expressed specific markers, ATP1a1, ZO\u20101 and CD56, indicative of CEC characteristics, aligning with the recapitulation of differentiation when forming a homogenous monolayer at the same level of isolated CECs without in vitro culture. These findings suggested that SCAP\u2010CM administration could be useful for future preclinical and clinical applications.<\/jats:p>","DOI":"10.1111\/wrr.13161","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T09:55:31Z","timestamp":1709114131000},"page":"292-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The proliferative effects of <scp>stem cells from apical papilla<\/scp>\u2010conditioned medium on rat corneal endothelial cells"],"prefix":"10.1111","volume":"32","author":[{"given":"Kimia","family":"Rahimiyan","sequence":"first","affiliation":[{"name":"ACECR Institute of Higher Education, Isfahan Branch  Isfahan Iran"},{"name":"Department of Animal Biotechnology Cell Science Research Center, Royan Institute for Biotechnology, ACECR  Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hossein","family":"Nasr 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