{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:08:18Z","timestamp":1783105698549,"version":"3.54.6"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The intricate relationship between regeneration and microbiota has recently gained attention, spanning diverse model organisms. Axolotl (<jats:italic>Ambystoma mexicanum<\/jats:italic>) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite its significance, a noticeable gap exists in understanding the interplay between axolotl regeneration and its microbiome. Here, we analyse in depth bacterial 16S rRNA amplicon dataset that we reported before as data resource and profile fungal community by sequencing ITS amplicons at the critical stages of limb regeneration (0\u20131\u20134\u20137\u201330\u201360\u2009days post amputation, \u2018dpa\u2019). Results reveal a decline in richness and evenness in the course of limb regeneration, with bacterial community richness recovering beyond 30\u2009dpa unlike fungi community. Beta diversity analysis reveals precise restructuring of the bacterial community along the three phases of limb regeneration, contrasting with less congruent changes in the fungal community. Temporal dynamics of the bacterial community highlight prevalent anaerobic bacteria in initiation phase and <jats:italic>Flavobacterium<\/jats:italic> bloom in the early phase correlating with limb blastema proliferation. Predicted functional analysis mirrors these shifts, emphasising a transition from amino acid metabolism to lipid metabolism control. Fungal communities shift from <jats:italic>Blastomycota<\/jats:italic> to <jats:italic>Ascomycota<\/jats:italic> dominance in the late regeneration stage. Our findings provide ecologically relevant insights into stage specific role of microbiome contributions to axolotl limb regeneration.<\/jats:p>","DOI":"10.1111\/wrr.13207","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T08:59:50Z","timestamp":1722934790000},"page":"826-839","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Temporal microbiome changes in axolotl limb regeneration: Stage\u2010specific restructuring of bacterial and fungal communities with a <scp><i>Flavobacterium<\/i><\/scp> bloom during blastema proliferation"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6068-3892","authenticated-orcid":false,"given":"Hanne","family":"Alt\u0131n","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Istanbul Medipol University International School of Medicine  Istanbul 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