{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:18:24Z","timestamp":1788455904907,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006286","name":"Kongelig Hofbuntmager Aage Bangs Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytokines are commonly deregulated in venous leg ulcers. We have investigated cytokine stability by incubating sterile\u2010filtered wound fluids from chronic venous leg ulcers in vitro. Incubation of wound fluids for 24\u2009h at 37\u00b0C decreased IL\u20101\u03b2 levels by 88% and TNF\u2010\u03b1 levels by 64%. IL\u20101\u03b2 was degraded by serine proteinases and metalloproteinases while the mechanism for reduced TNF\u2010\u03b1 remains elusive. The levels of the other peptides did not change significantly (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). Normal human dermal fibroblasts exposed to five of the six wound fluids showed increased proliferation with the length of prior incubation using an assay optimised for evaluation of wound fluid bioactivity. Exogenous IL\u20101\u03b2 and TNF\u2010\u03b1 unexpectedly increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) cell proliferation at concentrations that were measured in the wound fluids. In conclusion, the stability of the eight investigated cytokines in wound fluids differed and presumably the loss of detrimental factors, unlikely IL\u20101\u03b2 or TNF\u2010\u03b1, resulted in increased fibroblast proliferation.<\/jats:p>","DOI":"10.1111\/wrr.13210","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T06:34:51Z","timestamp":1725258891000},"page":"704-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cytokine stability in chronic wound fluid and its association to fibroblast proliferation"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0630-2694","authenticated-orcid":false,"given":"Ewa Anna","family":"Burian","sequence":"first","affiliation":[{"name":"Department of Dermato\u2010Venereology and Copenhagen Wound Healing Center, Bispebjerg Hospital University of Copenhagen  Copenhagen 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Bispebjerg Hospital University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"},{"name":"Digestive Disease Center, Bispebjerg Hospital University of Copenhagen  Copenhagen 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