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As one of the most abundant skin\u2010commensal,\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    is known as beneficial for the host, however, some strains can form biofilms and hinder wound healing. In this study,\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    distribution in VLUs and associated resistome were analysed in ulcer tissue from patients. Virulence of\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    isolates from VLUs were evaluated by whole genome sequencing, antimicrobial susceptibility testing, in vitro biofilm and binding assays, and assessment of biofilm\u2010forming capability and pro\u2010inflammatory potential using human ex vivo wound model. We demonstrated that\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    isolates from VLUs inhibit re\u2010epithelialization through biofilm\u2010dependent induction of IL\u20101\u03b2, IL\u20108, and IL\u20106 which was in accordance with impaired healing outcomes observed in patients. High extracellular matrix binding ability of VLU isolates was associated with antimicrobial resistance and expression levels of the\n                    <jats:italic>embp<\/jats:italic>\n                    and\n                    <jats:italic>sdrG<\/jats:italic>\n                    , responsible for bacterial binding to fibrinogen and fibrin, respectively. Finally, we showed that\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    from VLUs demonstrate pathogenic features with ability to impair healing which underscores the emergence of treatment\u2010resistant virulent lineages in patients with chronic ulcers.\n                  <\/jats:p>","DOI":"10.1111\/wrr.13231","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T02:23:41Z","timestamp":1729650221000},"page":"799-810","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multi\u2010drug resistant\n                    <i>Staphylococcus epidermidis<\/i>\n                    from chronic wounds impair healing in human wound model"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5275-4531","authenticated-orcid":false,"given":"Miroslav","family":"Dini\u0107","sequence":"first","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"},{"name":"Group for Probiotics and Microbiota\u2010Host Interaction, Institute of Molecular Genetics and Genetic Engineering University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Verpile","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie L.","family":"Burgess","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Ming","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Marjanovic","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen Nicole","family":"Beliz","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadar","family":"Lev\u2010Tov","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9341-0193","authenticated-orcid":false,"given":"Marjana","family":"Tomic\u2010Canic","sequence":"additional","affiliation":[{"name":"Wound Healing and Regenerative Medicine Research Program, Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0197-6198","authenticated-orcid":false,"given":"Irena","family":"Pastar","sequence":"additional","affiliation":[{"name":"Wound Healing and 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