{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:38:11Z","timestamp":1789148291741,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671914"],"award-info":[{"award-number":["81671914"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82472555"],"award-info":[{"award-number":["82472555"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bacterial colonisation in hypertrophic scars (HSs) has been reported, yet the precise mechanism of their contribution to scar formation remains elusive. To address this, we examined HS and normal skin (NS) tissues through Gram staining and immunofluorescence. We co\u2010cultured fibroblasts with heat\u2010inactivated <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S. aureus<\/jats:italic>) and evaluated their levels of apoptosis and proliferation by flow cytometry and Cell Counting Kit\u20108 assay, respectively. Additionally, we performed proteomic analysis and western blotting to identify upregulated proteins. To assess autophagy levels, we examined light chain 3 (LC3) expression through western blotting and immunofluorescence, and transmission electron microscopy (TEM) was performed to detect autophagy\u2010associated vesicles. Our results demonstrated a notable increase in bacterial load, primarily <jats:italic>S. aureus<\/jats:italic>, in HS tissues. Furthermore, <jats:italic>S. aureus<\/jats:italic> promoted fibroblast proliferation and enhanced the expression of profibrotic markers such as transforming growth factor \u03b21 (TGF\u2010\u03b21), vascular endothelial growth factor (VEGF), collagen I, collagen III and \u03b1 smooth muscle actin (\u03b1\u2010SMA). Proteomic analysis highlighted heat shock factor\u2010binding protein 1 (HSBP1) as a key upregulated protein mediating the profibrotic effects induced by <jats:italic>S. aureus<\/jats:italic>. Knockdown of HSBP1 reversed these effects. Intriguingly, HSBP1 also upregulated LC3 and Beclin\u20101 expression and increased the number of autophagosomes in fibroblasts. Finally, when fibroblasts stimulated by <jats:italic>S. aureus<\/jats:italic> were treated with HSBP1 siRNA, autophagy levels decreased significantly. Collectively, our findings suggest that <jats:italic>S. aureus<\/jats:italic>, via HSBP1, stimulates fibroblast proliferation and promotes their transition into myofibroblasts, triggering autophagy and fibrosis. These results underscore the potential of HSBP1 as a therapeutic target for the management of HSs.<\/jats:p>","DOI":"10.1111\/wrr.13253","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T11:55:51Z","timestamp":1737114951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bacteria in hypertrophic scars promote scar formation through <scp>HSBP1<\/scp>\u2010mediated autophagy"],"prefix":"10.1111","volume":"33","author":[{"given":"Bo","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Burn, Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Burn, Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai 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