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Pressure injuries, in particular, represent a serious problem for patients who are elderly or have limited mobility, such as wheelchair users or those who spend most of the day in bed. These injuries often result from prolonged pressure exerted on the skin over the bone. Treatment of pressure injuries is complex and costly. Emerging evidence suggests that the pressure injury microbiome plays a vital role in chronic wound formation and delaying wound healing. Additionally, antibiotics often fail due to the formation of resistant biofilms and the emergence of antimicrobial\u2010resistant bacteria. In this review, we will summarise the current knowledge on: (a) biofilms and microbiomes in pressure injuries; (b) in vitro and in vivo model systems to study pressure injuries, and (c) current therapies and novel treatment approaches. Understanding the complex interactions between microbes and the host immune system in pressure injuries will provide valuable insights to improve patient outcomes.<\/jats:p>","DOI":"10.1111\/wrr.70005","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T07:44:27Z","timestamp":1739519067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Pressure injuries and biofilms: Microbiome, model systems and therapies"],"prefix":"10.1111","volume":"33","author":[{"given":"Fahad","family":"Kabir","sequence":"first","affiliation":[{"name":"Department of Health Sciences Carleton University  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4086-3869","authenticated-orcid":false,"given":"Deborah Bow Yue","family":"Yung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of 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