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Many DFUs fail to heal completely and a major challenge includes identifying non\u2010healers early in treatment. However, effective predictive biomarkers for DFUs have not yet been validated. The goal of this study was to validate if two previously identified, objective and quantitative tissue biomarkers, c\u2010Myc and phosphorylated glucocorticoid receptor (p\u2010GR), could predict complete healing at week 12 in a multicenter observational cohort study of individuals with open DFUs, conducted by the NIDDK Diabetic Foot Consortium (DFC). Wound tissue collected at the initial visit was analysed for c\u2010Myc and p\u2010GR biomarkers by immunohistochemistry, quantifying their nuclear presence in full thickness epidermis and correlating with week 12 clinical outcomes. The primary analyses included AUC comparisons to assess the biomarkers' predictive capability for wound healing. Other analyses included descriptive measures and\n                    <jats:italic>t<\/jats:italic>\n                    \u2010tests to evaluate the difference between biomarker quantification among healers and non\u2010healers. Of 140 DFUs enrolled, 107 participants completed biomarker and clinical outcome data for analysis. The distributions of baseline c\u2010Myc and p\u2010GR between healed and not\u2010healed DFUs by week 12 were not significantly different (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Although the two biomarkers did not yield significant predictability (\u0394AUC\u2009=\u2009\u22120.006, 95% CI (\u22120.02, 0.01) and \u0394AUC\u2009=\u2009\u22120.0002, 95% CI (\u22120.01, 0.01),\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.025) for each of c\u2010Myc and p\u2010GR respectively, this first DFC clinical study using a national consortium of DFU centres successfully created a unique resource of wound\u2010related biomaterials coupled with the clinical outcomes, providing a platform for further biomarker discovery and validation.\n                  <\/jats:p>","DOI":"10.1111\/wrr.70044","type":"journal-article","created":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T05:56:06Z","timestamp":1749102966000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of c\u2010Myc and Phosphorylated Glucocorticoid Receptor (p\u2010\n                    <scp>GR<\/scp>\n                    ) for Predicting Diabetic Foot Ulcer Healing\u2014A Diabetic Foot Consortium Study"],"prefix":"10.1111","volume":"33","author":[{"given":"Robert S.","family":"Kirsner","sequence":"first","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0197-6198","authenticated-orcid":false,"given":"Irena","family":"Pastar","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Krambrink","sequence":"additional","affiliation":[{"name":"Data Coordinating Center of the Diabetic Foot Consortium, SABER Unit of Department of Biostatistics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadar","family":"Lev\u2010Tov","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie L.","family":"Burgess","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giselle","family":"Kolenic","sequence":"additional","affiliation":[{"name":"Data Coordinating Center of the Diabetic Foot Consortium, SABER Unit of Department of Biostatistics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Jozic","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Catanuto","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Marjanovic","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa L. Z.","family":"Jones","sequence":"additional","affiliation":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter X.\u2010K.","family":"Song","sequence":"additional","affiliation":[{"name":"Data Coordinating Center of the Diabetic Foot Consortium, SABER Unit of Department of Biostatistics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Clinical Research Unit of the Diabetic Foot Consortium, Department of Internal Medicine University of Michigan Medical School  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodica","family":"Pop\u2010Busui","sequence":"additional","affiliation":[{"name":"Clinical Research Unit of the Diabetic Foot Consortium, Department of Internal Medicine University of Michigan Medical School  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal M.","family":"Holmes","sequence":"additional","affiliation":[{"name":"Clinical Research Unit of the Diabetic Foot Consortium, Department of Internal Medicine University of Michigan Medical School  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathie","family":"Spino","sequence":"additional","affiliation":[{"name":"Data Coordinating Center of the Diabetic Foot Consortium, SABER Unit of Department of Biostatistics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9341-0193","authenticated-orcid":false,"given":"Marjana","family":"Tomic\u2010Canic","sequence":"additional","affiliation":[{"name":"Biomarker Analysis Unit of the Diabetic Foot Consortium, Wound Healing and Regenerative Medicine Research Program, Dr Phillip Frost Department of 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A Post\u2010Mortem Study of 130 Cases","volume":"79","author":"Villa A.","year":"1988","journal-title":"Minerva Medica"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12752"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.2337\/db20-0188"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02298-5"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202154558"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1275612"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36665-z"}],"container-title":["Wound Repair and Regeneration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.70044","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T07:10:49Z","timestamp":1763190649000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/wrr.70044"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/wrr.70044"],"URL":"https:\/\/doi.org\/10.1111\/wrr.70044","archive":["Portico"],"relation":{},"ISSN":["1067-1927","1524-475X"],"issn-type":[{"value":"1067-1927","type":"print"},{"value":"1524-475X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70044"}}