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Conventional treatments have limited efficacy. The non\u2010invasive use of low\u2010dose light treatments, termed photobiomodulation (PBM), has shown therapeutic benefits in diabetic patients. This study aimed to explore the response of endothelial cells to PBM treatment under hyperglycemic conditions in\u00a0vitro. The major goal was to gain mechanistic insights into the biological effects of low\u2010dose light, with the aim of optimising clinical treatment strategies. Therefore, human umbilical vein endothelial cells were exposed to hyperglycemic conditions (150\u2013300\u2009mM glucose) and incubated at 37\u00b0C with 5% CO<jats:sub>2<\/jats:sub> for 24\u2009h. The cells were then treated with low\u2010dose light (660\u2009nm, CW, 10\u2009mW\/cm<jats:sup>2<\/jats:sup>, 200\u2009s and 0.84 Einstein). Cell responses were assessed through key signalling pathways, evaluating proliferation using the AlamarBlue assay, migration through the wound scratch assay and angiogenesis via the tubulogenesis assay, with assessments after 24 or 48\u2009h. Data were analysed using one\u2010way ANOVA followed by Tukey's post\u2010test. Data showed that PBM treatments performed under controlled thermal conditions significantly improved endothelial cell proliferation, migration and tubulogenesis under hyperglycemic conditions. Crosstalk among platelet\u2010derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF\u2010\u03b21) signalling modulated these critical responses involving matrix metalloproteinases (MMP\u20102 and 9) activity. These findings showed that PBM treatments exert positive endothelial cell responses under hyperglycemic conditions that could contribute to improved diabetic wound healing. These observations provide mechanistic insights into enabling PBM as a novel and adjacent therapy for diabetic wound management.<\/jats:p>","DOI":"10.1111\/wrr.70068","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:30:01Z","timestamp":1753183801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Endothelial Cell Responses to Photobiomodulation Treatments in Diabetic Wounds Are Mediated via Concerted <scp>PDGF<\/scp>, <scp>VEGF<\/scp> and <scp>TGF<\/scp>\u2010\u03b2 Signalling"],"prefix":"10.1111","volume":"33","author":[{"given":"Vict\u00f3ria Regina","family":"da Silva Oliveira","sequence":"first","affiliation":[{"name":"Oral Biology, Surgery, &amp; Biomedical Engineering Departments University at Buffalo  Buffalo New York USA"},{"name":"Anatomy Department Institute of Biomedical Science of University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ridham","family":"Varsani","sequence":"additional","affiliation":[{"name":"Oral Biology, Surgery, &amp; Biomedical Engineering Departments University at Buffalo  Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahjuba","family":"Zehra","sequence":"additional","affiliation":[{"name":"Oral Biology, Surgery, &amp; Biomedical Engineering Departments University at Buffalo  Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Squarzoni","family":"Dale","sequence":"additional","affiliation":[{"name":"Anatomy Department Institute of Biomedical Science of University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Surgical Technique Department, Faculty of Medicine University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6116-2340","authenticated-orcid":false,"given":"Praveen","family":"Arany","sequence":"additional","affiliation":[{"name":"Oral Biology, Surgery, &amp; Biomedical Engineering Departments University at Buffalo  Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"IDF Diabetes Atlas","author":"Magliano D. 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