{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:31:02Z","timestamp":1777948262186,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Venous leg ulcers (VLUs) are chronic wounds in the lower limbs that cause significant morbidity. The underlying biology underpinning non\u2010healing in VLUs is still poorly understood and differences in transcriptomic profiles may help elucidate biological pathways involved in wound chronicity. A systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta\u2010Analyses. Studies were included if transcriptomes from adults with confirmed VLU were described. A search of EMBASE, OVID and PubMed between 1 January 1994 and 17 August 2024 yielded 429 articles; 34 studies were included after full\u2010text screening by two independent reviewers. Twenty\u2010nine studies investigated coding messenger RNA (mRNA) expression, while five studies investigated non\u2010coding RNA. Samples were obtained from wound biopsies (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200931) or whole blood (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093). There were variations in mRNA expression across comparisons between patients with VLUs and controls such as tissue from other patients, tissue from different sites and treated tissues. Altered levels of mRNA transcripts suggest chronic inflammatory states, hyperproliferation and reduced wound healing. Studies exploring non\u2010coding RNAs described their roles in regulating wound repair. Paired analyses of microRNA expression demonstrate impact on RNA expression profiles, with effects on inhibition of wound healing. Transcriptomic analysis provides new insights into the pathophysiology of the development and healing of VLUs. Further development of RNA biomarkers and gene expression profiles can improve diagnosis and prognostication of VLUs, but heterogeneity of current data may make it difficult to draw clinical significance.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration:<\/jats:bold>\n                    PROSPERO: CRD42024580526\n                  <\/jats:p>","DOI":"10.1111\/wrr.70140","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:22:23Z","timestamp":1773012143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomics in Venous Leg Ulcers (\n                    <scp>VLU<\/scp>\n                    ): A Systematic Review"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9756-1394","authenticated-orcid":false,"given":"Chien Lin","family":"Soh","sequence":"first","affiliation":[{"name":"Section of Vascular Surgery, Department of Surgery and Cancer Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5789-0353","authenticated-orcid":false,"given":"Kia Hau Matthew","family":"Tan","sequence":"additional","affiliation":[{"name":"Section of Vascular Surgery, Department of Surgery and Cancer Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alun Huw","family":"Davies","sequence":"additional","affiliation":[{"name":"Section of Vascular Surgery, Department of Surgery and Cancer Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Onida","sequence":"additional","affiliation":[{"name":"Section of Vascular Surgery, Department of Surgery and Cancer Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvsv.2022.11.005"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/000331979704800111"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800810206"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2014.23.12.601"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2013.22.2.58"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvsv.2014.04.006"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.13366"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2020.1381"},{"issue":"11","key":"e_1_2_12_10_1","article-title":"Venous Leg Ulcers: Advanced Therapies and New Technologies","volume":"9","author":"Aleksandrowicz H.","year":"2021","journal-title":"Biomedicine"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12570"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12814"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X14568677"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16353-8"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1801214"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0268355516688357"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n71"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n160"},{"key":"e_1_2_12_19_1","article-title":"Transcriptomics in Venous Leg Ulcers: A Systematic Review","author":"Soh C. 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