{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:31:03Z","timestamp":1777948263306,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acute trauma wounds with exposed bone or tendon present significant reconstructive challenges, with high rates of dermal regeneration template (DRT) failure, often due to missed infections. Standard\u2010of\u2010care wound assessment, which relies on subjective clinical signs and traditional swabbing methods, frequently fails to detect clinically significant bacterial loads (&gt;\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009CFU\/g). This prospective observational study evaluated the ability of intraoperative bacterial fluorescence imaging to predict DRT outcomes in patients with complex wounds. A subset of 13 patients underwent a blinded fluorescence imaging procedure whereby modified Levine and fluorescence\u2010guided cultures were collected immediately prior to DRT placement. We found a 63% overall DRT complication rate, with 56% of patients experiencing partial DRT failure despite being clinically and microbiologically cleared of infection pre\u2010operatively. Fluorescence imaging demonstrated high predictive accuracy; DRTs placed on fluorescence\u2010negative areas had an 85.6% success rate, whereas those placed on fluorescence\u2010positive areas had a success rate of only 14.4%. Fluorescence\u2010guided cultures detected pathogens more often than traditional methods. These findings suggest that fluorescence imaging shows potential value as an adjunctive tool for real\u2010time, intraoperative bacterial detection, which can guide more effective wound bed preparation and significantly improve DRT integration rates.\n                  <\/jats:p>","DOI":"10.1111\/wrr.70142","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T06:29:27Z","timestamp":1776148167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial Fluorescence Signals Predict Dermal Regeneration Template Integration Outcomes"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7405-6312","authenticated-orcid":false,"given":"Carlos Daniel Torres","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Plastic and Reconstructive Surgery Department Hospital General Dr Rub\u00e9n Le\u00f1ero  M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9465-6590","authenticated-orcid":false,"given":"Erik","family":"Hanson\u2010Viana","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department Hospital General Dr Rub\u00e9n Le\u00f1ero  M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hecly Lya Vazquez","family":"Morales","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department Hospital General Dr Rub\u00e9n Le\u00f1ero  M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Manzali","family":"Flores","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department Hospital General Dr Rub\u00e9n Le\u00f1ero  M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0b013E31802CB83F"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2021.0146"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.14080"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhm.688"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/GOX.0000000000002869"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ta.0000195982.71400.84"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004630-200301000-00009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/SAP.0000000000003909"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2019-0279"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2020.1272"},{"key":"e_1_2_9_12_1","first-page":"11","article-title":"Rapid Diagnosis of \n                     pseudomonas aeruginosa\n                   in Wounds With Point\u2010Of\u2010Care Fluorescence Imaing","volume":"11","author":"Raizman R.","year":"2021","journal-title":"Diagnostics (Basel)"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.70678"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2019.28.12.824"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc21-2218"},{"key":"e_1_2_9_16_1","first-page":"10","article-title":"Routine Fluorescence Imaging to Detect Wound Bacteria Reduces Antibiotic Use and Antimicrobial Dressing Expenditure While Improving Healing Rates: Retrospective Analysis of 229 Foot Ulcers","volume":"10","author":"Price N.","year":"2020","journal-title":"Diagnostics (Basel)"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2021.30.Sup2.S18"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/21621918251359558"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2022.0095"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2024.04.003"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-197803000-00007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics9010022"},{"key":"e_1_2_9_23_1","first-page":"14","article-title":"Determination of the Course of Cyan Fluorescence of \n                     pseudomonas aeruginosa\n                   With a Handheld Bacterial Imaging Device","volume":"14","author":"Pham E.","year":"2024","journal-title":"Diagnostics (Basel)"},{"key":"e_1_2_9_24_1","first-page":"395","article-title":"Osteomyelitis: Diagnosis and Treatment","volume":"104","author":"Bury D. C.","year":"2021","journal-title":"American Family Physician"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1070311"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12717"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2004.01221.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1693401"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2013.11.034"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2010.00670.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.13737"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jbcr\/irae046"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2022.115831"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2005.01764.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000006609"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000111883.93604.85"}],"container-title":["Wound Repair and Regeneration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.70142","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/wrr.70142","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.70142","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T06:16:58Z","timestamp":1777789018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/wrr.70142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/wrr.70142"],"URL":"https:\/\/doi.org\/10.1111\/wrr.70142","archive":["Portico"],"relation":{},"ISSN":["1067-1927","1524-475X"],"issn-type":[{"value":"1067-1927","type":"print"},{"value":"1524-475X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70142"}}