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Adipose\u2010derived mesenchymal stromal cells (ASCs) represent a promising therapeutic option; however, their regenerative potential is limited under hyperglycaemic conditions. This study investigated whether metformin could enhance ASC function and improve their therapeutic potential for tissue regeneration. Transcriptomic analysis of wound tissue identified pathways associated with angiogenesis, inflammation, extracellular matrix remodelling and cell survival, including PI3K\u2013AKT, JAK\u2013STAT and AGE\u2013RAGE signalling. Functionally, metformin increased ASC proliferation in a concentration\u2010dependent manner and promoted sustained cell expansion over time. Metformin\u2010treated ASCs also enhanced fibroblast migration in a co\u2010culture system, indicating improved paracrine pro\u2010reparative signalling. Metformin further increased the secretion of angiogenic factors, including vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF), supporting a pro\u2010angiogenic secretory profile. Metformin enhanced CD31 expression and tube formation capacity of ASCs under normoglycemic conditions, particularly in combination with VEGF; however, these effects were attenuated under high\u2010glucose conditions. Additionally, metformin improved ASC adhesion to small intestinal submucosa (SIS) scaffolds and supported endothelial marker expression in a three\u2010dimensional biomaterial environment. Collectively, these findings demonstrate that metformin promotes a pro\u2010regenerative ASC phenotype characterized by enhanced proliferation, paracrine signalling, endothelial\u2010associated responses and scaffold integration. However, hyperglycaemic conditions partially impair these effects, highlighting the influence of metabolic stress on regenerative responses. Overall, metformin preconditioning represents a promising strategy to enhance ASC\u2010based therapies for chronic and diabetic wound healing.<\/jats:p>","DOI":"10.1111\/wrr.70214","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:54:42Z","timestamp":1791345282000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metformin Enhances the Pro\u2010Regenerative Function of Human Adipose\u2010Derived Stromal Cells for Diabetic Wound Healing"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8818-6118","authenticated-orcid":false,"given":"Kristina Lee","family":"Khaw","sequence":"first","affiliation":[{"name":"Cooper Medical School of Rowan University  Camden New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2504-7450","authenticated-orcid":false,"given":"Max","family":"von 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