{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:49:23Z","timestamp":1791622163113,"version":"4.3.4"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Wound Repair Regeneration"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Healing after split\u2010skin grafting remains difficult to anticipate in small\u2010area burns, particularly when wounds show no clinically diagnosed infection. We tested whether the burn wound microbiome before grafting captures early wound states linked to subsequent healing. Twenty\u2010six adults with mid\u2010dermal to full\u2010thickness burns involving &lt;\u200910% total body surface area underwent longitudinal sampling at surgery, graft check and clinic review. At surgery, swabs were collected from the burn wound before grafting, the split\u2010skin donor site and anatomically matched uninjured control skin. Across 170 infection\u2010free samples, microbiome profiling showed that pre\u2010grafting burn wounds already had reduced microbial diversity and distinct community structure compared with matched control skin. This baseline dysbiosis included depletion of commensal\u2010associated anaerobes, including\n                    <jats:italic>Finegoldia<\/jats:italic>\n                    and\n                    <jats:italic>Anaerococcus<\/jats:italic>\n                    and enrichment of opportunistic burn\u2010associated taxa, including\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    and\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    . Donor sites acquired a related wound\u2010like microbiome and remodelled in parallel with burn wounds during healing, but post\u2010grafting burn microbiota were not preferentially similar to matched donor sites, arguing against a donor\u2010to\u2010burn microbial transfer. The clearest clinical signal was present before grafting: lower burn wound diversity at surgery was associated with delayed healing, and baseline community structure differed in wounds that later required regrafting. These findings suggest that the pre\u2010grafting burn wound microbiome may provide an early, infection\u2010independent marker of healing trajectory and should be tested as a predictive biomarker in larger prospective cohorts.\n                  <\/jats:p>","DOI":"10.1111\/wrr.70217","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:24:27Z","timestamp":1791620667000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Early Burn Wound Microbiome, Not Donor\u2010Site Transfer, Marks Graft Healing Outcome"],"prefix":"10.1111","volume":"34","author":[{"given":"Elad","family":"Zvi","sequence":"first","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"},{"name":"Department of Surgery, School of Translational Medicine Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Chi","sequence":"additional","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4145-2385","authenticated-orcid":false,"given":"Thinh Sieu","family":"Tang","sequence":"additional","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sriya","family":"Bobba","sequence":"additional","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Neely","sequence":"additional","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxanne","family":"Chatzis","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes S.","family":"Kern","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine Monash University  Melbourne Australia"},{"name":"Department of Dermatology Bayside Health (The Alfred)  Melbourne Australia"},{"name":"SkIIN Discovery Program Bayside Health (The Alfred) and Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2787-3317","authenticated-orcid":false,"given":"Shiva","family":"Akbarzadeh","sequence":"additional","affiliation":[{"name":"Victorian Adult Burns Service Bayside Health (The Alfred)  Melbourne Australia"},{"name":"Department of Surgery, School of Translational Medicine Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9920-1181","authenticated-orcid":false,"given":"C\u00e9line","family":"Pattaroni","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Translational Medicine Monash University  Melbourne Australia"},{"name":"SkIIN Discovery Program Bayside Health (The Alfred) and Monash University  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2016.08.013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/2321\u20103868.143620"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD009715\/full"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0000000000000355"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2537"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000874"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12632"},{"issue":"1","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"10667","DOI":"10.1038\/s41598-021-89822-z","article-title":"Longitudinal Profiling of the Burn Patient Cutaneous and Gastrointestinal Microbiota: A Pilot Study","volume":"11","author":"Lima K. M.","year":"2021","journal-title":"Scientific Reports"},{"issue":"42","key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"eadw1410","DOI":"10.1126\/sciadv.adw1410","article-title":"Bacterial Communities Co\u2010Develop With Respiratory Immunity Early in Life, Linking Dysbiosis to Systemic Monocyte Signature and Wheezing","volume":"11","author":"Pattaroni C.","journal-title":"Science Advances"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.19719006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/s40168-018-0605-2","article-title":"Simple Statistical Identification and Removal of Contaminant Sequences in Marker\u2010Gene and Metagenomics Data","volume":"6","author":"Davis N. M.","year":"2018","journal-title":"Microbiome"},{"issue":"1","key":"e_1_2_8_15_1","first-page":"352","article-title":"Using DECIPHER v2.0 to Analyze Big Biological Sequence Data in R","volume":"8","author":"Wright E. S.","year":"2016","journal-title":"R Journal"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq706"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1038\/nmeth.2658","article-title":"Differential Abundance Analysis for Microbial Marker\u2010Gene Surveys","volume":"10","author":"Paulson J. N.","year":"2013","journal-title":"Nature Methods"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1654\u20101103.2003.tb02228.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010022\u201002655\u20105"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106483"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2009.024"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2021.02.014"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/GOX.0000000000006174"}],"container-title":["Wound Repair and Regeneration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.70217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/wrr.70217","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/wrr.70217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:24:29Z","timestamp":1791620669000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/wrr.70217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/wrr.70217"],"URL":"https:\/\/doi.org\/10.1111\/wrr.70217","archive":["Portico"],"relation":{},"ISSN":["1067-1927","1524-475X"],"issn-type":[{"value":"1067-1927","type":"print"},{"value":"1524-475X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70217"}}