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Accordingly, achievement to suitable cell source with potential\u2010to\u2010long\u2010term survival and appropriate differentiation can be an effective step in this direction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After derivation of human adipose\u2010derived mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">HAD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s), they were stably transfected with a vector containing Turbo\u2010green fluorescent protein (<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">JR<\/jats:styled-content>ed to be able to trace them after transplantation. Labeled <jats:styled-content style=\"fixed-case\">HAD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were transplanted into the intact adult rat eye and their survival, integration, and migration during 6\u00a0months post\u2010transplantation were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The transplanted cells were traceable in the rat vitreous humor (<jats:styled-content style=\"fixed-case\">VH<\/jats:styled-content>) up until 90\u00a0days after transplantation, with gradual reduction in numbers, their adhesion and expansion capacity after recovery. These cells were also integrated into the ocular tissues. Nonetheless, some of the implanted cells succeeded to cross the blood\u2013retina barrier (<jats:styled-content style=\"fixed-case\">BRB<\/jats:styled-content>) and accumulate in the spleen with time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The survival of the <jats:styled-content style=\"fixed-case\">HAD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s for a period of 90\u00a0days in <jats:styled-content style=\"fixed-case\">VH<\/jats:styled-content> and even longer period of up to 6\u00a0months in other eye tissues makes them a promising source to be considered in regenerative medicine of eye diseases. However, the potency of crossing the <jats:styled-content style=\"fixed-case\">BRB<\/jats:styled-content> by the implanted cells suggests that use of <jats:styled-content style=\"fixed-case\">HAD<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s must be handled with extreme caution.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12033","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T02:17:53Z","timestamp":1368757073000},"page":"165-176","source":"Crossref","is-referenced-by-count":28,"title":["Human adipose\u2010derived mesenchymal stem cells can survive and integrate into the adult rat eye following xenotransplantation"],"prefix":"10.1111","volume":"20","author":[{"given":"Aliakbar","family":"Haddad\u2010Mashadrizeh","sequence":"first","affiliation":[{"name":"Cell and Molecular Biotechnology Research Group Institute of Biotechnology Ferdowsi University of Mashhad Mashhad Iran"},{"name":"Department of 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