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Baboon recipients of life\u2010supporting Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content> kidney (n\u00a0=\u00a07) or heterotopic heart (n\u00a0=\u00a014) grafts received either no immunosuppression (n\u00a0=\u00a04), or one of several partial or full immunosuppressive regimens (n\u00a0=\u00a017). Fourteen additional baboons received a Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">hCPRP<\/jats:styled-content> kidney (n\u00a0=\u00a05) or heart (n\u00a0=\u00a09) and similar treatment regimens. Immunologic, pathologic, and coagulation parameters were measured at frequent intervals. <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> of Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content> organs occurred in 9\/21 baboons (43%). <jats:styled-content style=\"fixed-case\">hCPRP<\/jats:styled-content> expression reduced the Gal<jats:styled-content style=\"fixed-case\">TKO EGF<\/jats:styled-content> incidence to 7% (1\/14; P\u00a0&lt;\u00a00.01 vs. Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content> alone). At 30 mins, complement deposits were more intense in organs in which <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> developed (P\u00a0&lt;\u00a00.005). The intensity of peri\u2010transplant platelet activation (as \u03b2\u2010thromboglobulin release) correlated with <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>, as did the cumulative coagulation score (P\u00a0&lt;\u00a00.01). We conclude that (i) the transgenic expression of a <jats:styled-content style=\"fixed-case\">hCPRP<\/jats:styled-content> on the vascular endothelium of a Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content> pig reduces the incidence of <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> and reduces complement deposition, (ii) complement deposition and platelet activation correlate with early Gal<jats:styled-content style=\"fixed-case\">TKO<\/jats:styled-content> organ failure, and (iii) the expression of a <jats:styled-content style=\"fixed-case\">hCPRP<\/jats:styled-content> reduces <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content> but does not prevent systemic coagulation activation. Additional strategies will be required to control coagulation activation.<\/jats:p>","DOI":"10.1111\/xen.12176","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T21:20:30Z","timestamp":1436908830000},"page":"310-316","source":"Crossref","is-referenced-by-count":96,"title":["Early graft failure of GalTKO pig organs in baboons is reduced by expression of a human complement pathway\u2010regulatory protein"],"prefix":"10.1111","volume":"22","author":[{"given":"Agnes M.","family":"Azimzadeh","sequence":"first","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean S.","family":"Kelishadi","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed B.","family":"Ezzelarab","sequence":"additional","affiliation":[{"name":"Thomas E. Starzl Transplantation Institute University of Pittsburgh Pittsburgh PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avneesh K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Research Program NHLBI\/NIH Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Stoddard","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayato","family":"Iwase","sequence":"additional","affiliation":[{"name":"Thomas E. Starzl Transplantation Institute University of Pittsburgh Pittsburgh PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianshu","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Burdorf","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Sievert","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Avon","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangfei","family":"Cheng","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Ayares","sequence":"additional","affiliation":[{"name":"Revivicor Blacksburg VA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith A.","family":"Horvath","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Research Program NHLBI\/NIH Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip C.","family":"Corcoran","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Research Program NHLBI\/NIH Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad M.","family":"Mohiuddin","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery Research Program NHLBI\/NIH Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf N.","family":"Barth","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine and Baltimore VAMC Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K. 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