{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:31:40Z","timestamp":1778668300637,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T00:00:00Z","timestamp":1436918400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatocyte transplantation is a promising therapy for acute liver failure. Cell therapy using xenogeneic sources has emerged as an alternative treatment for patients with organ failure due to the shortage of transplantable human organs. The purpose of this study was to improve the survival of mice with acute liver failure by transplanting encapsulated neonatal pig re\u2010aggregated liver cells (<jats:styled-content style=\"fixed-case\">NPRLC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver injury was induced in C57\/<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>6 male mice by the injection of 600\u00a0mg\/kg of acetaminophen. Xenogeneic liver cells were isolated from a neonatal pig and processed via re\u2010aggregation and encapsulation to improve the efficiency of the xenogeneic liver cell transplantation. The neonatal pig liver showed abnormal lobule structure. Isolated cells were re\u2010aggregated and intraperitoneally transplanted into acute liver failure mice models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Re\u2010aggregated cells showed significantly enhanced viability and significantly greater synthesis of albumin and urea than cells cultured in monolayers. Further, we observed improved serum levels of <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content>, and the survival rate of mice with acute liver failure was improved by the intraperitoneal transplantation of encapsulated hepatocytes (48\u00a0000 equivalent (Eq) per mouse).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study shows that using encapsulated <jats:styled-content style=\"fixed-case\">NPRLC<\/jats:styled-content>s improves the efficacy of xenogeneic liver cell transplantation for the treatment of mice with acute liver failure. Therefore, this may be a good strategy for bridge therapy for the treatment of acute liver failure in humans.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12177","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T06:41:40Z","timestamp":1436942500000},"page":"249-259","source":"Crossref","is-referenced-by-count":15,"title":["Successful xenotransplantation with re\u2010aggregated and encapsulated neonatal pig liver cells for treatment of mice with acute liver failure"],"prefix":"10.1111","volume":"22","author":[{"given":"Dong\u2010Sik","family":"Ham","sequence":"first","affiliation":[{"name":"Department of Endocrinology &amp; Metabolism College of Medicine The Catholic University of Korea Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min\u2010Sang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Endocrinology &amp; Metabolism College of Medicine The Catholic University of Korea Seoul 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