{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T11:46:18Z","timestamp":1789818378304,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T00:00:00Z","timestamp":1468972800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["TRR 127"],"award-info":[{"award-number":["TRR 127"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Xenotransplantation using pig cells, tissues or organs may be associated with the transmission of porcine zoonotic micro\u2010organisms. Hepatitis E virus (<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>), porcine cytomegalovirus (<jats:styled-content style=\"fixed-case\">PCMV<\/jats:styled-content>) and porcine endogenous retroviruses (<jats:styled-content style=\"fixed-case\">PERV<\/jats:styled-content>s) are potentially zoonotic micro\u2010organisms which do not show clinical symptoms in pigs and which are due to the low expression level difficult to detect. G\u00f6ttingen Minipigs (G\u00f6<jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>) are often used for biomedical investigations and they are well characterized concerning the presence of numerous bacteria, fungi, viruses and parasites and therefore may be used for islet cell transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Islet cells derived from three G\u00f6<jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>were transplanted into four healthy, non\u2010diabetic cynomolgus monkeys using a macroencapsulation device.<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, nested<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, real\u2010time<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and Western blot analyses were used to estimate the presence of<jats:styled-content style=\"fixed-case\">PERV<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">PCMV<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>in the donors and recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using sensitive detection methods, no<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>was found in the donor pigs and in the pig islet cell preparations. Antibodies against<jats:styled-content style=\"fixed-case\">PERV<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">PCMV<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>were not found in all cynomolgus monkeys with exception of one monkey showing an immune response against<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>. Using real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, no<jats:styled-content style=\"fixed-case\">PCMV<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>were found in the sera of all monkeys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although the donor islet cells and the recipients were negative for<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>using<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and Western blot analysis, in one recipient, antibodies against<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>were found, indicating infection in a single case. All recipients were negative for antibodies against<jats:styled-content style=\"fixed-case\">PERV<\/jats:styled-content>, and all were negative for<jats:styled-content style=\"fixed-case\">PCMV<\/jats:styled-content>, indicating absence of infection. As<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>was not detected in the donor pig before transplantation, a more complex and regular screening of the animals using highly sensitive methods is required to avoid virus transmission.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12252","type":"journal-article","created":{"date-parts":[[2016,7,21]],"date-time":"2016-07-21T04:12:44Z","timestamp":1469074364000},"page":"320-327","source":"Crossref","is-referenced-by-count":33,"title":["Islet cell transplantation from G\u00f6ttingen minipigs to cynomolgus monkeys: analysis of virus safety"],"prefix":"10.1111","volume":"23","author":[{"given":"Vladimir A.","family":"Morozov","sequence":"first","affiliation":[{"name":"HIV and other Retroviruses Robert Koch Institute Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Visceral\u2010 Thorax\u2010 and Vascular Surgery University Hospital Carl Gustav Carus Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Medicine III University Hospital Carl Gustav Carus Dresden Germany"},{"name":"Paul Langerhans Institute Dresden of Helmholtz Centre Munich at University Clinic Carl Gustav Carus of TU Dresden and DZD\u2010 German Centre for Diabetes Research Dresden Germany"},{"name":"Centre for Regenerative Therapies Dresden Technische Universit\u00e4t Dresden Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avi","family":"Rotem","sequence":"additional","affiliation":[{"name":"Beta\u2010O<sub>2<\/sub> Technologies Ltd. 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