{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:46:15Z","timestamp":1753879575104,"version":"3.41.2"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,4,11]],"date-time":"2017-04-11T00:00:00Z","timestamp":1491868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004530","name":"Universiti Putra Malaysia","doi-asserted-by":"publisher","award":["04\u201001\u201013\u20101202FR"],"award-info":[{"award-number":["04\u201001\u201013\u20101202FR"]}],"id":[{"id":"10.13039\/501100004530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dead islets replaced with viable islets are a promising offer to restore normal insulin production to a person with diabetes. The main reason for establishing a new islet source for transplantation is the insufficiency of human donor pancreas while using xenogeneic islets perhaps assists this problem. The expression of <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 is essential for the pancreas expansion. In mature \u03b2\u2010cells, <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 has several critical roles such as glucose sensing, insulin synthesis, and insulin secretion. In this study, we aimed to evaluate the expression of pancreatic duodenal homeobox\u20101 (<jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1) in treated caprine islets in culture and to assess the protective effects of antioxidant factors on the <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 gene in cultured caprine islets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Purified islets were treated with serum\u2010free, serum, <jats:styled-content style=\"fixed-case\">IBMX<\/jats:styled-content>, tocopherol, or <jats:styled-content style=\"fixed-case\">IBMX<\/jats:styled-content> and tocopherol media. Quantitative polymerase chain reaction and Western blotting were carried out to compare the expression levels of <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 in treated purified islets cultured with different media.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Islets treated with <jats:styled-content style=\"fixed-case\">IBMX<\/jats:styled-content>\/tocopherol exhibited the highest fold change in the relative expression of <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 on day 5 post\u2010treatment (relative expression: 6.80\u00b12.08), whereas serum\u2010treated islets showed the lowest fold changes in <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 expression on day 5 post\u2010treatment (0.67\u00b10.36), as compared with the expression on day 1 post\u2010treatment. Insulin production and viability tests of purified islets showed superiority of islet at supplemented serum\u2010free media with <jats:styled-content style=\"fixed-case\">IBMX<\/jats:styled-content>\/tocopherol compared to other cultures (53.875%\u00b11.59%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results indicated that supplemented serum\u2010free medium with tocopherol and <jats:styled-content style=\"fixed-case\">IBMX<\/jats:styled-content> enhances viability and <jats:styled-content style=\"fixed-case\">PDX<\/jats:styled-content>1 gene expression compared to serum\u2010added and serum\u2010free media.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12302","type":"journal-article","created":{"date-parts":[[2017,4,11]],"date-time":"2017-04-11T08:26:40Z","timestamp":1491899200000},"source":"Crossref","is-referenced-by-count":4,"title":["Improvement of isolated caprine islet survival and functionality in vitro by enhancing of <scp>PDX<\/scp>1 gene expression"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4590-8704","authenticated-orcid":false,"given":"Homayoun","family":"Hani","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Faculty of Veterinary Medicine Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeenathul Nazariah","family":"Allaudin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Faculty of Veterinary Medicine Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd\u2010Azmi","family":"Mohd\u2010Lila","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Faculty of Veterinary Medicine Universiti Putra Malaysia  Serdang Selangor 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