{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:21:08Z","timestamp":1790619668128,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,24]],"date-time":"2017-09-24T00:00:00Z","timestamp":1506211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671844"],"award-info":[{"award-number":["81671844"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Corneal disease is the second most common blinding disease in the world. The shortage of cornea donors has become the greatest challenge in curing corneal disease. Decellularized porcine corneas have the potential to be clinically applied as a substitute for human cornea in lamellar keratoplasty. Porcine corneas will help relieve the cornea donor shortage. To comprehensively evaluate the characteristics of the grafts and the effect of the decellularized porcine cornea on the host cornea after clinical transplantation, we assessed the microstructure of the transplanted decellularized porcine corneal tissues. Through the analysis of the microstructure of the tissues by H&amp;E staining, <jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content> and immunofluorescence of anti\u2010human vimentin, anti\u2010pig vimentin,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20103, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108, <jats:styled-content style=\"fixed-case\">INF<\/jats:styled-content>\u2010\u03b3, and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 immunofluorescence staining, we found that despite the slight rejection that occurred, the porcine cornea has good biocompatibility and can provide a scaffold for cell growth. Genetic analysis using Solexa sequencing of the samples showed that decellularized porcine corneas cannot affect genes in patients\u2019 corneas. Decellularized porcine corneas are effective biological materials for use in corneal transplantation.<\/jats:p>","DOI":"10.1111\/xen.12338","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T04:16:51Z","timestamp":1506313011000},"source":"Crossref","is-referenced-by-count":33,"title":["Comprehensive evaluation of decellularized porcine corneal after clinical transplantation"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5375-2765","authenticated-orcid":false,"given":"Yan","family":"Shi","sequence":"first","affiliation":[{"name":"Eye Hospital First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timur","family":"Bikkuzin","sequence":"additional","affiliation":[{"name":"Eye Hospital First Affiliated Hospital of Harbin Medical University  Harbin China"},{"name":"Central Laboratory of Scientific Research Bashkir State Medical University  Ufa Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Song","sequence":"additional","affiliation":[{"name":"Eye Hospital First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Jin","sequence":"additional","affiliation":[{"name":"Eye Hospital First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Eye Hospital First Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113843"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60437-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181f237ef"},{"key":"e_1_2_6_5_1","first-page":"161","article-title":"Focus on the clinical application of the first artificial bioengineered cornea in China","volume":"52","author":"Shi WY","year":"2016","journal-title":"Chinese journal of ophthalmology"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.04.025"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2007.04.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0b013e328344870c"},{"key":"e_1_2_6_9_1","first-page":"613","article-title":"Effect of heterogenic corneal stroma transplantation on peripheral T cells of rats","volume":"20","author":"Luo XL","year":"2004","journal-title":"Chinese Journal of Pathophysiology"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00148-4"},{"key":"e_1_2_6_11_1","first-page":"165","article-title":"Biomechanical characterization of decellularized and cross\u2010linked bovine pericardium","volume":"16","author":"Oswal D","year":"2007","journal-title":"J Heart Valve Dis"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-7273"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2010.01174.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2011.02261.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13096"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.26.5.313.15440"},{"key":"e_1_2_6_17_1","first-page":"535","article-title":"Relative quantitative analysis of corneal immunogenicity","volume":"38","author":"Wang Z","year":"2002","journal-title":"Chinese journal of ophthalmology"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-08-0699"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.13.2455"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.111.13.1897"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.05.045"},{"key":"e_1_2_6_22_1","first-page":"977","article-title":"Human corneal interleukin\u20108, IL\u20101 and TNF\u2010induced gene expression and secretion","volume":"139","author":"Elner VM","year":"1991","journal-title":"The American journal of pathology"},{"key":"e_1_2_6_23_1","first-page":"165","article-title":"Phenotype, specificity, and function of T cell subsets and T cell interactions involved in skin allograft rejection","volume":"5","author":"Rosenberg AS","year":"1987","journal-title":"J Exp Med"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fxen.12338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.12338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T04:44:43Z","timestamp":1694753083000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/xen.12338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,24]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1111\/xen.12338"],"URL":"https:\/\/doi.org\/10.1111\/xen.12338","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,24]]},"article-number":"e12338"}}