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This facility was populated with caesarian derived, colostrum deprived (<jats:styled-content style=\"fixed-case\">CDCD<\/jats:styled-content>) piglets, from sows of conventional\u2010specific (or specified) pathogen\u2010free (<jats:styled-content style=\"fixed-case\">SPF<\/jats:styled-content>) health status in six cohorts over a 10\u2010month period. In all cases,<jats:styled-content style=\"fixed-case\">CDCD<\/jats:styled-content>piglets fulfilled<jats:styled-content style=\"fixed-case\">DPF<\/jats:styled-content>status including negativity for porcine circovirus (<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>), a particularly environmentally robust and difficult to inactivate virus which at the time of<jats:styled-content style=\"fixed-case\">SAF<\/jats:styled-content>population was epidemic in the<jats:styled-content style=\"fixed-case\">US<\/jats:styled-content>commercial swine production industry. Two outbreaks of<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>infection were subsequently detected during sentinel testing. The first occurred several weeks after<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u2010negative animals were moved under quarantine from the nursery into an animal holding room. The apparent origin of<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>was newly installed stainless steel penning, which was not sufficiently degreased thereby protecting viral particles from disinfection. The second outbreak was apparently transmitted via employee activities in the Caesarian\u2010section suite adjacent to the barrier facility. In both cases,<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>was contained in the animal holding room where it was diagnosed making a complete facility depopulation\u2010repopulation unnecessary.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Infectious<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>was eliminated during both outbreaks by the following: euthanizing infected animals, disposing of all removable items from the affected animal holding room, extensive cleaning with detergents and degreasing agents, sterilization of equipment and rooms with chlorine dioxide, vaporized hydrogen peroxide, and potassium peroxymonosulfate, and for the second outbreak also glutaraldehyde\/quaternary ammonium. Impact on other barrier animals throughout the process was monitored by frequent<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>diagnostic testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>After close monitoring for 6\u00a0months indicating<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>absence from all rooms and animals, herd animals were removed from quarantine status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ten years after<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>clearance following the second outbreak, due to strict adherence to biosecurity protocols and based on ongoing sentinel diagnostic monitoring (currently monthly), the herd remains<jats:styled-content style=\"fixed-case\">DPF<\/jats:styled-content>including<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>negative.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12428","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T10:18:43Z","timestamp":1534587523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Pathogen elimination and prevention within a regulated, Designated Pathogen Free, closed pig herd for long\u2010term breeding and production of xenotransplantation materials"],"prefix":"10.1111","volume":"25","author":[{"given":"Jeske","family":"Noordergraaf","sequence":"first","affiliation":[{"name":"Spring Point Project Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienne","family":"Schucker","sequence":"additional","affiliation":[{"name":"Spring Point Project Minneapolis MN 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