{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:45:20Z","timestamp":1792154720117,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T00:00:00Z","timestamp":1534550400000},"content-version":"vor","delay-in-days":48,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC127"],"award-info":[{"award-number":["CRC127"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>For clinical xenotransplantation, transplants must be free of porcine cytomegalovirus (PCMV). Piglets become infected primarily in the perinatal period by the mother sow. While individual donor animals can be protected from infection by isolation husbandry, success is not guaranteed and this strategy poses the risk of undetected infections and raises animal welfare questions. Here, we present the establishment of a completely PCMV\u2010negative pig herd for breeding donor animals for xenotransplantation. Eleven pregnant DanAvl Basic hybrid sows were purchased from a designated pathogen\u2010free (DPF), PCMV\u2010positive colony and transferred to a new pig facility at the Centre for Innovative Medical Models (CiMM) 4\u00a0weeks prior to farrowing. At the age of 24\u00a0hours, piglets were early\u2010weaned and transferred to a commercially available Rescue Deck system dedicated to motherless rearing of piglets. Sows were removed from the facility. The PCMV status of F1\u2010generation animals was determined at regular intervals over a period of 14\u00a0months by a sensitive real\u2010time PCR\u2010based detection method testing blood, nasal swabs and cultured peripheral blood mononuclear cells (PBMCs). F1 sows were used as recipients of genetically modified embryos to generate a xenotransplant donor herd. Offspring were tested for PCMV accordingly. All offspring have remained PCMV negative over the whole observation period of 14\u00a0months. A completely PCMV\u2010negative pig herd for xenotransplantation has thus been successfully established.<\/jats:p>","DOI":"10.1111\/xen.12449","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T10:18:43Z","timestamp":1534587523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Early weaning completely eliminates porcine cytomegalovirus from a newly established pig donor facility for xenotransplantation"],"prefix":"10.1111","volume":"25","author":[{"given":"Stefanie","family":"Egerer","sequence":"first","affiliation":[{"name":"Center for Innovative Medical Models (CiMM) Institute for Molecular Animal Breeding and Biotechnology LMU Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Fiebig","sequence":"additional","affiliation":[{"name":"Robert Koch Institute  Berlin 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