{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:47:19Z","timestamp":1753879639469,"version":"3.41.2"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T00:00:00Z","timestamp":1534464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2015R1D1A1A09083556","2016R1D1A1B03932507"],"award-info":[{"award-number":["2015R1D1A1A09083556","2016R1D1A1B03932507"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>A long\u2010segmental tracheal lesion is difficult to repair by tracheal allotransplantation due to the lack of a well\u2010defined blood supply for blood vessel anastomosis. The donor trachea needs to be revascularized within a well\u2010vascularized soft tissue flap for several months to allow successful trachea allotransplantation. To date, xenotransplantation using the wild\u2010type or genetically modified pig has been widely studied. The object of this study was to evaluate the feasibility of a small\u2010sized (2\u00a0\u00d7\u00a02\u00a0cm) wild\u2010type pig tracheal patchy in a dog tracheal defect model before trying a long\u2010segment tracheal defect model and using a genetically modified pig as a donor in dog xenotransplantation.<\/jats:p><\/jats:sec>Method<\/jats:title>Three healthy beagle dogs (8\u20109\u00a0kg) were used as recipients, and one pig (20\u00a0kg) was used as the donor. A pig cartilaginous tracheal patchy (2\u00a0\u00d7\u00a02\u00a0cm half tube) was sutured to the tracheal resected site in each dog. Antithymocyte globulin (2.5\u00a0mg\/kg infusion, D0 and 1), tacrolimus (4.5\u00a0mg\/kg, twice a day for 2\u00a0months), and methylprednisolone sodium succinate (1\u00a0mg\/kg, IV<\/jats:styled-content>, for 2\u00a0days and tapering) were administered for immunosuppression. The levels IL<\/jats:styled-content>\u20102 and IFN<\/jats:styled-content>\u2010\u03b3 in the serum were measured at D0, 7, and 28. Tracheoscopy was performed at D28, 60, and 90. The recipients were sacrificed at D90, and the expression of dog and pig genes in the graft was evaluated by PCR<\/jats:styled-content>. Histopathological examination of the graft was conducted.<\/jats:p><\/jats:sec>Results<\/jats:title>All of the dogs survived without complications during the experimental period. Their IL<\/jats:styled-content>\u20102 and IFN<\/jats:styled-content>\u2010\u03b3 levels were significantly increased at D7 after transplantation compared to D0 and D28 (P<\/jats:italic>\u00a0<\u00a00.05). The pig tracheal patchy site was open, and no stenosis was observed until D90 on tracheoscopy, when pale mucosa erosion was observed; there was also remnant suture material at D28. However, the tracheal patchy sites gradually became similar to normal mucosa at D60 and 90. The expression of pig genes was detected in the graft by PCR<\/jats:styled-content>. Normal epithelium and CD<\/jats:styled-content>3 cells were observed in the histological examination at D90.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In this study, our data suggest that the pig tracheal patchy can be successfully engrafted into the trachea of dog, although erosion of mucosa on the graft was seen at D30, in spite of the discordant species.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12452","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T09:51:13Z","timestamp":1534499473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pig tracheal patchy xenotransplantation in the dog"],"prefix":"10.1111","volume":"26","author":[{"given":"Tae\u2010Ki","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Veterinary Surgery Veterinary Medical Center and College of Veterinary Medicine Chungbuk National University Cheongju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0906-0117","authenticated-orcid":false,"given":"Jong\u2010Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Xenotransplantation Research Center Seoul Korea"},{"name":"Institute of Endemic Diseases Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok Hwa","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery Veterinary Medical Center and College of Veterinary Medicine Chungbuk National University Cheongju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e3283539673"},{"key":"e_1_2_8_3_1","first-page":"S186","article-title":"Tracheal replacement","volume":"8","author":"Delaere P","year":"2016","journal-title":"J Thorac Dis"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2014.12.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-017-0024-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.10.026"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2007.03.015"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3089.2005.00258.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/200521121063"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890230047010"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940511400406"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200205150-00010"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fxen.12452","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.12452","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T18:58:01Z","timestamp":1694717881000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/xen.12452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,17]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/xen.12452"],"URL":"https:\/\/doi.org\/10.1111\/xen.12452","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"type":"print","value":"0908-665X"},{"type":"electronic","value":"1399-3089"}],"subject":[],"published":{"date-parts":[[2018,8,17]]},"assertion":[{"value":"2018-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12452"}}