{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:49:54Z","timestamp":1768812594456,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,16]],"date-time":"2019-02-16T00:00:00Z","timestamp":1550275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue\u2010engineered heart valves aim to reproduce the biological properties of natural valves with anatomically correct structure and physiological performance. The closest alternative to creating an ideal heart valve substitute is to use decellularized porcine heart valves, due to their anatomy and availability. However, the immunological barrier and the structural maintenance limit the long\u2010term physiological performance of decellularized porcine heart valves. This study investigated the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) structure of aortic and pulmonary porcine valves decellularized by a low concentration sodium dodecyl sulfate (SDS)\u2010based method in order to determine the<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>scaffold (<jats:styled-content style=\"fixed-case\">ECMS<\/jats:styled-content>) conditions related to remodeling potential. To assess the structures of the leaflets and conduits of the heart valves,<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>components and their organization were evaluated by histology, biochemical analysis (<jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>), scanning electron microscopy, multiphoton microscopy, tensile test, immunofluorescence labeling (<jats:styled-content style=\"fixed-case\">IF<\/jats:styled-content>), and Raman microspectroscopy used to draw a profile of the cell niches. Histology and multiphoton imaging of decellularized aortic and pulmonary leaflets and conduits revealed a collagen and elastin histoarchitecture with rearrangement, loosening fibers, and glycosaminoglycan depletion confirmed by biochemistry quantification. The potential cytotoxicity of<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>residues was eliminated after 10 wash cycles. The mechanical properties of the structure of the valve indicated a functional resistance of decellularized<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>. The<jats:styled-content style=\"fixed-case\">IF<\/jats:styled-content>demonstrated the presence of basement membrane, suggesting a potential structure for host cell attachment. The<jats:styled-content style=\"fixed-case\">RM<\/jats:styled-content>analysis showed evidence of molecular interactions, suggesting conservation of the chemical composition, particularly among the protein molecular structures. The structural analyses performed in the semilunar porcine heart valves demonstrate that decellularized<jats:styled-content style=\"fixed-case\">ECMS<\/jats:styled-content>has structural properties that support physiological performance and potential host tissue integration. In fact, decellularized leaflet scaffolds were prone to cell interaction after human adipose\u2010derived stromal cell seeding and culturing. Further analysis of biocompatibility, particularly the<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>\u2010cell interaction, can elucidate the remodeling process, in preserved decellularized heart valve scaffold.<\/jats:p>","DOI":"10.1111\/xen.12503","type":"journal-article","created":{"date-parts":[[2019,2,16]],"date-time":"2019-02-16T08:50:49Z","timestamp":1550307049000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Structural assessments in decellularized extracellular matrix of porcine semilunar heart valves: Evaluation of cell niches"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-5837","authenticated-orcid":false,"given":"Jo\u00e3o Gabriel","family":"Roderjan","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Engenharia Biom\u00e9dica Universidade Tecnol\u00f3gica Federal do Paran\u00e1 Curitiba 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Loures","family":"Bueno","sequence":"additional","affiliation":[{"name":"Setor de Medicina Interna Universidade Federal do Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Diniz Affonso","family":"da Costa","sequence":"additional","affiliation":[{"name":"Escola de Medicina Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2016.05.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.10.080"},{"issue":"3","key":"e_1_2_7_4_1","first-page":"380","article-title":"PGA (polyglycolic acid)\u2010P4HB (poly\u20104\u2010hydroxybutyrate)\u2010based bioengineered valves in the rat aortic circulation","volume":"25","author":"Ksiazek AA","year":"2016","journal-title":"J Heart 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