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While the interaction of Neu5Gc and diet\u2010induced anti\u2010Neu5Gc Abs occurs in all normal individuals, endothelium activation by elicited anti\u2010Neu5Gc Abs following a challenge with animal\u2010derived materials, such as following xenotransplantation, had been postulated. Ten primary human EC preparations were cultured with affinity\u2010purified anti\u2010Neu5Gc Abs from human sera obtained before or after exposure to Neu5Gc\u2010glycosylated rabbit IgGs (elicited Abs). RNAs of each EC preparation stimulated in various conditions by purified Abs were exhaustively sequenced. EC transcriptomic patterns induced by elicited anti\u2010Neu5Gc Abs, compared with pre\u2010existing ones, were analyzed. qPCR, cytokines\/chemokines release, and apoptosis were tested on some EC preparations. The data showed that anti\u2010Neu5Gc Abs\u00a0induced 967 differentially expressed (DE) genes. Most DE genes are shared following EC activation by pre\u2010existing or anti\u2010human T\u2010cell globulin (ATG)\u2010elicited anti\u2010Neu5Gc Abs. Compared with pre\u2010existing anti\u2010Neu5Gc\u00a0Abs, which are normal component of ECs environment, elicited anti\u2010Neu5Gc Abs down\u2010regulated 66 genes, including master genes of EC function. Furthermore, elicited anti\u2010Neu5Gc Abs combined with complement\u2010containing serum down\u2010regulated most transcripts mobilized by serum alone. Both types of anti\u2010Neu5Gc Abs\u2010induced a dose\u2010 and complement\u2010dependent release of selected cytokines and chemokines. Altogether, these data show that, compared with pre\u2010existing anti\u2010Neu5Gc Abs, ATG\u2010elicited anti\u2010Neu5Gc Abs specifically modulate genes related to cytokine responses, MAPkinase cascades, chemotaxis, and integrins and do not skew the EC transcriptome toward a pro\u2010inflammatory profile in vitro.<\/jats:p>","DOI":"10.1111\/xen.12535","type":"journal-article","created":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T03:59:03Z","timestamp":1562817543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Elicited and pre\u2010existing anti\u2010Neu5Gc antibodies differentially affect human endothelial cells transcriptome"],"prefix":"10.1111","volume":"26","author":[{"given":"Ludmilla","family":"Le Berre","sequence":"first","affiliation":[{"name":"Centre de Recherche en Transplantation et Immunologie (CRTI), INSERM Universit\u00e9 de Nantes Nantes France"},{"name":"Institut de Transplantation Urologie N\u00e9phrologie (ITUN) CHU Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Danger","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Transplantation et Immunologie (CRTI), INSERM Universit\u00e9 de Nantes Nantes France"},{"name":"Institut de Transplantation Urologie N\u00e9phrologie (ITUN) CHU Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoa L.","family":"Mai","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Transplantation et Immunologie (CRTI), INSERM Universit\u00e9 de Nantes Nantes France"},{"name":"Institut de Transplantation Urologie N\u00e9phrologie (ITUN) CHU Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Amon","sequence":"additional","affiliation":[{"name":"Department of Cell Research and Immunology, The George S. 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