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Notably, MSCs maintained blood\u2010gas homeostasis in the first 24\u00a0hours and prevented FLF\u2010induced elevation of prothrombin time, international normalized ratio, and creatinine and ammonia levels in the first 3\u00a0days. With MSCs, serum levels of liver enzymes gradually decreased after 3\u00a0days, and platelet count was back to normal at 1\u00a0week of FLF. MSCs promoted liver regeneration within 2\u00a0weeks and differentiated into functional hepatocytes at 2\u20104\u00a0weeks after transplantation, evidenced by increase in Ki67\u2010positive cells, detectable human hepatocyte growth factor, human vascular endothelial growth factor, human hepatocyte\u2010specific antigen, and human albumin\u2010expressing cells in the liver at different time points. Reactive oxidative species (ROS) were accumulated after FLF and eliminated at 4\u00a0weeks after MSC transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Together, MSCs prolong the survival and prevent lethal sequelae of I\u2010R injury\u2010induced FLF by maintenance of liver\u2010function homeostasis and rescue of ROS in the acute stage and by homing and differentiation into hepatocytes in the subacute stage.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12542","type":"journal-article","created":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T11:55:29Z","timestamp":1561031729000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mesenchymal stem cells prolong survival and prevent lethal complications in a porcine model of fulminant liver 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