{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T21:28:22Z","timestamp":1782941302040,"version":"3.54.5"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP 148 899"],"award-info":[{"award-number":["MOP 148 899"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neonatal porcine islets (NPIs) are a promising tissue source for clinical islet xenotransplantation. To facilitate graft monitoring and recovery if needed, an extra\u2010hepatic transplant site would be optimal. In addition, islet transplantation into the portal vein has been associated with life\u2010threatening intraperitoneal bleeding, portal vein thrombosis, hepatic steatosis, and loss of islet graft function. Although it is hypoxic, the subcutaneous space is a potential extra\u2010hepatic location for clinical islet transplantation. In this study, we explore the benefits of fibrin scaffolds in enhancing the engraftment and long\u2010term function of NPI grafts in this ectopic site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Diabetic immune\u2010compromised mice were transplanted with 5000 NPIs under the kidney capsule (KC), and subcutaneously with or without fibrin (SC\u00a0+\u00a0F, SC, respectively). All mice were monitored for reversal of hyperglycemia and long\u2010term metabolic function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All mice transplanted with NPI under the KC or SC\u00a0+\u00a0F (12\/12 and 17\/17, respectively) achieved normal fasting blood glucose levels between 5 and 22\u00a0weeks post\u2010transplantation and displayed normal glucose tolerance during an intraperitoneal glucose tolerance test. In contrast, NPIs transplanted SC with no fibrin (n\u00a0=\u00a07) failed to obtain normoglycemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Fibrin matrix facilitates engraftment of NPIs in the subcutaneous site of diabetic mice. These data support further investigation of the subcutaneous site for clinical islet xenotransplantation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12575","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T09:00:40Z","timestamp":1575882040000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fibrin supports subcutaneous neonatal porcine islet transplantation without the need for pre\u2010vascularization"],"prefix":"10.1111","volume":"27","author":[{"given":"Bassem F.","family":"Salama","sequence":"first","affiliation":[{"name":"Alberta Diabetes Institute University of Alberta Edmonton Alberta Canada"},{"name":"Department of Surgery University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Seeberger","sequence":"additional","affiliation":[{"name":"Alberta Diabetes Institute University of Alberta Edmonton Alberta Canada"},{"name":"Department of Surgery University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-4351","authenticated-orcid":false,"given":"Gregory S.","family":"Korbutt","sequence":"additional","affiliation":[{"name":"Alberta Diabetes Institute University of Alberta Edmonton Alberta Canada"},{"name":"Department of Surgery University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.7.2060"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a007823"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2016.178"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc15-1988"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-013-0401-0"},{"key":"e_1_2_9_8_1","unstructured":"Collaborative Islet Transplant Registry (CITR) Coordinating Center and Investigators.2017; Tenth Annual Report.www.citregistry.org. 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