{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T13:13:57Z","timestamp":1782911637194,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970655 82070776 82270796"],"award-info":[{"award-number":["81970655 82070776 82270796"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ30946"],"award-info":[{"award-number":["2021JJ30946"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The murine heterotopic cardiac transplantation model has been widely used to study antigen\u2010specific immune responses or new immunosuppressive agents, which have a strong correlation with peripheral lymph nodes. Thus, a new organ transplantation model that is applicable to related studies is needed. Here, we describe a groin\u2010site murine heart transplantation model using a cuff technique, in which the donor aorta and pulmonary artery are anastomosed to the truncated femoral vessels of the recipient. The mean survival time (MST) of the grafts in BALB\/c\u2010to\u2010C57BL\/6 allo\u2010transplant group was 7.2 \u00b1 0.3 days, and 1.9 \u00b1 0.2 days in BALB\/c\u2010to\u2010Sprague\u2010Dawley (SD) rat xeno\u2010transplant group. H&amp;E results show that donor hearts from both groups demonstrate typical pathological features at the endpoint. Evans Blue tracing revealed that the popliteal lymph nodes of the grafted side hindlimb are larger than those of the contralateral side. Moreover, IHC staining for CD3, CD20 shows that the germinal center and cortex region of the grafted side of popliteal lymph nodes is apparently increased than that of the contralateral side. To sum up, this model may serve as an ideal model to study the role of peripheral lymph nodes in organ transplant rejection. In addition, extra\u2010peritoneal grafting makes a step forward in animal welfare under the 3Rs\u2019 principle (Replacement, Reduction, Refinement).<\/jats:p>","DOI":"10.1111\/xen.12817","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T08:54:39Z","timestamp":1691398479000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A murine groin site cardiac transplantation model\u2014applicable tool for studying roles of peripheral lymph nodes in transplantation"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3502-6086","authenticated-orcid":false,"given":"Yaguang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjia","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingda","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Qi","sequence":"additional","affiliation":[{"name":"Peking University Health Science Center Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinguo","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xubiao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hedong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Organ Transplantation The Fifth Clinical Medical College of Henan University of Chinese Medicine (Zhengzhou People's Hospital) Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longkai","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helong","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Kidney Transplantation The Second Xiangya Hospital of Central South University Changsha China"},{"name":"Clinical Research Center for Organ Transplantation in Hunan Province Central South University Changsha China"},{"name":"Clinical Immunology Center Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015495"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11948-015-9659-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1964.01320040061009"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199112000-00035"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199104000-00031"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198511000-00019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2010.03388.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.11.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04082-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(01)00154-5"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201744428"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12331"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15831"},{"issue":"160","key":"e_1_2_8_15_1","article-title":"Optimization of the cuff technique for murine heart transplantation","author":"Ma Y","year":"2020","journal-title":"J Vis 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