{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:56:32Z","timestamp":1792457792964,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Porcine tissues display a great potential as donor tissues in xenotransplantation, including cell therapy. Cryopreserving clinical grade porcine tissue and using it as a source for establishing therapeutic cells should be advantageous for transportation and scheduled manufacturing of MSCs. Of note, we previously performed encapsulated porcine islet transplantation for the treatment of unstable type 1 diabetes mellitus in the clinical setting. It has been reported that co\u2010transplantation of islets and Mesenchymal stem cells (MSCs) enhanced efficacy. We assume that co\u2010transplantation of porcine islets and porcine islet\u2010derived MSCs could improve the efficacy of clinical islet xenotransplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MSCs were established from fresh and cryopreserved non\u2010clinical grade neonatal porcine islets and bone marrow (termed non\u2010clinical grade npISLET\u2010MSCs and npBM\u2010MSCs, respectively), as well as from cryopreserved clinical grade neonatal porcine islets (termed clinical grade npISLET\u2010MSCs). Subsequently, the cell proliferation rate and diameter, surface marker expression, adipogenesis, osteogenesis, and colony\u2010forming efficiency of the MSCs were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell proliferation rate and diameter did not differ between clinical grade and non\u2010clinical grade npISLET\u2010MSCs. However, non\u2010clinical grade npBM\u2010MSCs were significantly shorter and smaller than both npISLET\u2010MSCs (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). MSC markers (CD29, CD44, and CD90) were strongly expressed in clinical grade npISLET\u2010MSCs and non\u2010clinical grade npISLET\u2010MSCs and npBM\u2010MSCs. The expression of MSC\u2010negative markers CD31, CD34, and SLA\u2010DR was low in all MSCs. Clinical grade npISLET\u2010MSCs derived from adipose and osteoid tissues were positive for Oil Red and alkaline phosphatase staining. The results of colony\u2010forming assay were not significantly different between clinical grade npISLET\u2010MSCs and non\u2010clinical grade npBM\u2010MSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The method described herein was successful in of developing clinical grade npISLET\u2010MSCs from cryopreserved islets. Cryopreserved clinical grade porcine islets could be an excellent stable source of MSCs for cell therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/xen.12831","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T11:57:40Z","timestamp":1697543860000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and characterization of islet\u2010derived mesenchymal stem cells from clinical grade neonatal porcine cryopreserved islets"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8063-0293","authenticated-orcid":false,"given":"Takeshi","family":"Kikuchi","sequence":"first","affiliation":[{"name":"Research and Development Center Otsuka Pharmaceutical Factory, Inc.  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