{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:00Z","timestamp":1787016180779,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3404304"],"award-info":[{"award-number":["2023YFC3404304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2024AFB581"],"award-info":[{"award-number":["2024AFB581"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Induction therapy is necessary to prevent acute rejection of xenografts. At present, basiliximab is rarely used as an induction therapy agent in solid organ xenotransplantation. In this study, we conducted in vitro experiments to investigate the inhibitory effect of basiliximab on human anti\u2010porcine xenogeneic immune responses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A xenogeneic and allogeneic mixed lymphocyte reaction (MLR) system was established using peripheral blood mononuclear cells (PBMCs) isolated from GTKO\/CMAHKO\/\u03b24GalNT2KO (triple\u2010knockout, TKO) pigs or humans as stimulator cells, and another group of human PBMCs as responder cells. Various concentrations of basiliximab were added to the MLR systems as interventions. The inhibitory effects of basiliximab on the proliferation and cytokine production of human T cells were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PBMCs from TKO pigs or humans stimulated significant proliferation of human T cells. Basiliximab inhibited human CD4\n                      <jats:sup>+<\/jats:sup>\n                      and CD8\n                      <jats:sup>+<\/jats:sup>\n                      T\u2010cell proliferation in a typical dose\u2010dependent manner in both xenogeneic and allogeneic MLR. When the concentration of basiliximab reached 1 \u00b5g\/mL, the proliferation rates of xenoreactive CD4\n                      <jats:sup>+<\/jats:sup>\n                      and CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells decreased by more than 72%, which was quite similar to the effect in the allogeneic MLR. The inhibitory effects of basiliximab on xenogeneic T\u2010cell responses were further confirmed by the detection of CD25 expression and supernatant cytokines (IFN\u2010\u03b3, TNF\u2010\u03b1), and the results were similar to those for allogeneic MLR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Basiliximab can significantly reduce the xenoreactivity of human lymphocytes against TKO pig cells, and its inhibitory effect is no less than that on allogeneic T cell responses, supporting its potential as induction therapy in xenotransplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/xen.70101","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T11:10:13Z","timestamp":1764933013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Evaluation of Basiliximab as an Induction Therapy for Xenotransplantation"],"prefix":"10.1111","volume":"32","author":[{"given":"Hao","family":"Feng","sequence":"first","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosheng","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Organ Transplantation Ministry of Education National Health Commission Chinese Academy of Medical Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahui","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Organ Transplantation Ministry of Education National Health Commission Chinese Academy of Medical Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengke","family":"Pan","sequence":"additional","affiliation":[{"name":"Chengdu Clonorgan Biotechnology Co., LTD  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Organ Transplantation Ministry of Education National Health Commission Chinese Academy of Medical Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0574-9785","authenticated-orcid":false,"given":"Gang","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Key Laboratory of Organ Transplantation Ministry of Education National Health Commission Chinese Academy of Medical Sciences Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2023"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006594\u20104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010022\u201000624\u20106"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejmoa2120238"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002796"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047679\u20106"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12516"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003622"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4635.The"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002804"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060068"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15712"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2165\/11203990\u2010000000000\u201000000"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)32187\u20100"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16876"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12876"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20106143.2011.03509.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20106143.2012.04031.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15329"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000296832.92128.94"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402140"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495\u2010200969110\u201000007"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/xen.70101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T23:00:55Z","timestamp":1785452455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/xen.70101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/xen.70101"],"URL":"https:\/\/doi.org\/10.1111\/xen.70101","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"2025-07-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70101"}}